Anti-CAD Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P27708

Core Product Specifications and Parameters

Biological Background: CAD Function and Localization

• CAD is a multifunctional protein that carries out the first three enzymatic activities of the de novo pyrimidine biosynthesis pathway: carbamoylphosphate synthetase (CPSase), aspartate transcarbamylase (ATCase), and dihydroorotase (DHOase).
• The CPSase function occurs in two steps: a glutamine-dependent amidotransferase (GATase) domain generates ammonia, which is then used by the ammonium-dependent carbamoyl phosphate synthetase domain to produce carbamoyl phosphate from ATP and hydrogencarbonate.
• ATCase catalyzes the formation of carbamoyl-L-aspartate from L-aspartate and carbamoyl phosphate.
• DHOase catalyzes the cyclization of carbamoyl aspartate to dihydroorotate, completing the initial steps of UMP synthesis.
• CAD localizes to both the cytoplasm and the nucleus.
• It is subject to acetylation and phosphorylation, and functions as a zinc- and metal-dependent allosteric enzyme.
• Mutations in CAD are associated with congenital disorder of glycosylation type 1Z (CDG1Z) and developmental and epileptic encephalopathy 50 (DEE50/EIEE50).
• Alternative names for CAD include Carbamoyl phosphate synthetase 2-aspartate transcarbamylase-dihydroorotase.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 1650-1900) of human CAD; consider this when interpreting data in truncation or isoform studies.
• For western blotting, expect a band at approximately 243 kDa; use appropriate gel percentage and transfer conditions for high-molecular-weight proteins.
• In IHC-P and IF/ICC, both cytoplasmic and nuclear staining may be observed; include appropriate controls and optimize fixation/permeabilization.
• Validate the antibody in your specific sample type and application by running a dilution series, as optimal working concentrations can vary.
• Cross-reactivity with mouse and rat is reported; confirm performance in these species if they are part of your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-CAD polyclonal antibody is a reliable reagent for studying pyrimidine metabolism, metabolic disorders, and associated epilepsy or glycosylation defects. Beyond this product, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing support for rare targets to accelerate your diagnostic development.