Anti-CACYBP Polyclonal Antibody (KO Validated) - Q9HB71

Core Product Specifications and Parameters

Biological Background: CACYBP Function and Localization

• CACYBP (Calcyclin-binding protein), also known as S100A6-binding protein and Siah-interacting protein, is encoded by the CACYBP gene. UniProt accessions: Q9HB71.
• It may function as a molecular bridge in ubiquitin E3 complexes, potentially linking calcium signaling to ubiquitin-dependent proteasomal degradation.
• Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1), implicating it in Wnt pathway regulation and related cellular processes.
• Subcellular localization includes both the nucleus and cytoplasm, consistent with its roles in signaling and protein degradation.
• Post-translational modifications include phosphorylation and acetylation, as identified by proteomics, suggesting regulation by multiple upstream pathways.
• Alternative splicing generates multiple isoforms, which may contribute to functional diversity or tissue-specific regulation.
• Contains a structured domain (3D-structure available) and belongs to the reference proteome, with direct protein sequencing evidence supporting its existence.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human CACYBP (residues 1–228), which may provide broad epitope coverage for detection across species.
• For Western blot, use whole cell lysates and expect a band near 26 kDa; consider using a knockout-validated lysate as a negative control to confirm specificity.
• For IF/ICC, standard fixation with 4% PFA and permeabilization with 0.1% Triton X-100 may be suitable; optimize based on your specific cell type.
• ELISA can be used for quantitative detection; pair with a matched coating antigen if possible, and validate with recombinant protein standards.
• Endogenous CACYBP may be expressed at low levels in some tissues; enrichment or loading high amounts of lysate could improve signal.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For targets like CACYBP involved in ubiquitination and beta-catenin signaling, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your assay development. Partner with us to bridge your research with reliable, scalable supply solutions.