Anti-CACYBP Polyclonal Antibody for WB, ELISA - Q9HB71

Core Product Specifications and Parameters

Biological Background: Calcyclin-binding Protein Function and Localization

• Calcyclin-binding protein (CACYBP), also known as S100A6-binding protein and Siah-interacting protein, is encoded by the CACYBP gene (alternative symbols: S100A6BP, SIP), producing multiple isoforms via alternative splicing.
• It plays a central role in calcium-dependent ubiquitination and subsequent proteasomal degradation, likely serving as a molecular bridge within ubiquitin E3 complexes.
• CACYBP participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1), linking calcium signaling to canonical Wnt pathway regulation.
• Localizes to both the nucleus and cytoplasm, enabling its proteolytic functions across cellular compartments.
• Exhibits post-translational modifications including acetylation and phosphorylation, supported by direct protein sequencing and proteomic identification.
• Annotated with keywords such as Ubl conjugation pathway, alternative splicing, and 3D-structure availability, underscoring its regulatory complexity.

Experimental Guidance and Technical Tips

• For Western blot, consider running appropriate positive and negative controls (e.g., lysates from CACYBP-expressing tissues or cell lines) and validate with knockdown or knockout samples to confirm specificity.
• The antibody’s reported cross-reactivity with mouse and rat makes it suitable for rodent model studies; titrate and validate in your specific sample system.
• For ELISA, optimize antigen coating conditions and antibody dilution to achieve optimal signal-to-noise ratios.
• As a polyclonal antibody, lot-to-lot variability may occur; it is advisable to titrate each new lot and include internal reference standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports your ubiquitination and calcium signaling research by providing one-stop access to validated IVD raw materials like anti-CACYBP antibodies. Our supply capabilities extend to optimized monoclonal/polyclonal pairs, bulk ancillary reagents, and rare target sourcing—helping diagnostic developers and labs streamline procurement from concept to clinic.