Anti-CACNG1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q06432

Core Product Specifications and Parameters

Biological Background: CACNG1 Function and Localization

• The CACNG1 gene encodes the voltage-dependent calcium channel gamma-1 subunit, a regulatory component of the L-type calcium channel complex in skeletal muscle.
• As a dihydropyridine-sensitive L-type calcium channel subunit, CACNG1 modulates channel inactivation kinetics, shaping the amplitude and duration of calcium currents.
• The protein is specifically expressed at the sarcolemma (cell membrane) of skeletal muscle fibers, consistent with its role in excitation-contraction coupling.
• CACNG1 is a multi-pass transmembrane glycoprotein with disulfide bonds, characteristic of ion channel auxiliary subunits.
• Key structural features include four transmembrane helices, intracellular N- and C-termini, and an extracellular loop housing glycosylation sites.
• The gamma subunit family (CACNG1-8) shares a common topology but exhibits tissue-specific expression; CACNG1 is uniquely restricted to skeletal muscle.

Experimental Guidance and Technical Tips

• The immunogen targets the N-terminal extracellular region (aa 30-110), which may allow detection of the mature protein in its native conformation or under denaturing conditions.
• For Western blot, consider using a low-percentage gel to resolve the 25 kDa band; heating samples in reducing buffer is recommended to break disulfide bonds.
• For immunofluorescence/immunocytochemistry, permeabilization may be required to access the intracellular epitope; validate staining in appropriate positive control like skeletal muscle tissue or myotubes.
• Due to the polyclonal nature, the antibody may exhibit broad reactivity; confirm specificity by performing a blocking peptide control or using knockout samples.
• Optimize working dilutions empirically for each application and sample type (human, mouse, rat) to achieve optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research labs to streamline procurement of IVD raw materials, technical services, and consulting. This anti-CACNG1 antibody supports skeletal muscle calcium channel research and assay development. As a one-stop sourcing bridge, we offer validated antibody pairs, bulk monoclonal/polyclonal antibodies, ancillary reagents, and custom sourcing for rare targets — helping you move from concept to clinic with a single, reliable supply partner.