Anti-CACNA2D3 Rabbit Polyclonal Antibody for WB, ELISA - Q8IZS8

Core Product Specifications and Parameters

Biological Background: CACNA2D3 Function and Localization

• CACNA2D3, also known as voltage-gated calcium channel subunit alpha-2/delta-3, is the alpha-2/delta-3 subunit of voltage-dependent calcium channels.
• It regulates calcium current density and activation/inactivation kinetics of the calcium channel, acting as a regulatory subunit for P/Q-type (CACNA1A), N-type (CACNA1B), and L-type (CACNA1C or CACNA1D) channels, but not T-type (CACNA1G).
• At the protein level, CACNA2D3 is only detected in brain, with no expression in lung, testis, aorta, spleen, jejunum, ventricular muscle, and kidney. Related references: PMID:11687876 PMID:11245980
• The protein is a membrane protein with a signal peptide, transmembrane helix, and multiple disulfide bonds, glycosylation, and phosphorylation sites.
• Keywords from UniProt include alternative splicing, calcium channel, calcium transport, glycoprotein, ion channel, ion transport, metal-binding, phosphoprotein, and voltage-gated channel, indicating diverse functions and modifications.
• The CACNA2D3 gene is a member of the CACNA2D family, which comprises four genes encoding auxiliary subunits of voltage-gated calcium channels.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 900-1040 of human CACNA2D3. This region may be present in full-length and certain splice variants; consider validating antibody specificity with sequence alignment and appropriate positive controls.
• For Western blotting, the predicted molecular weight is ~123 kDa. Use a suitable gel percentage (e.g., 6-8% SDS-PAGE) and optimize transfer conditions for high-molecular-weight proteins.
• Since CACNA2D3 is brain-specific, use brain tissue lysates or relevant cell lines to confirm antibody performance in WB.
• For ELISA applications, titrate the antibody to determine the optimal working concentration for your assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, offering this anti-CACNA2D3 rabbit polyclonal antibody for research into ion channels and neurological disorders. In addition to individual antibodies, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support rare-target raw-material procurement to streamline your diagnostic development from concept to clinic.