Anti-CACNA1S Polyclonal Antibody for IF-P and ELISA - Q13698

Core Product Specifications and Parameters

Biological Background: CACNA1S Function and Localization

• CACNA1S, also known as Cav1.1, is the pore-forming alpha-1S subunit of the voltage-gated L-type calcium channel in skeletal muscle.
• It mediates L-type calcium currents and is critical for excitation-contraction coupling by interacting with RYR1 to trigger Ca(2+) release from the sarcoplasmic reticulum.
• This channel belongs to the high-voltage activated (HVA) group of calcium channels.
• CACNA1S is specifically localized to the sarcolemma and T-tubule membranes of skeletal muscle cells.
• Expression is restricted to skeletal muscle tissue.
• The protein contains multiple transmembrane domains, is glycosylated, and is regulated by calmodulin binding and phosphorylation.
• Mutations in CACNA1S are associated with diseases such as hypokalemic periodic paralysis and malignant hyperthermia susceptibility.

Experimental Guidance and Technical Tips

• For immunofluorescence (IF-P), consider using paraformaldehyde-fixed cells or tissue sections, and validate antibody specificity with appropriate controls such as knockdown or knockout models.
• In ELISA applications, this polyclonal antibody may be used for detection of the immunogen peptide or recombinant protein; optimize coating and blocking conditions to minimize background.
• Although validated for IF-P and ELISA, if exploring Western blotting, note the high molecular weight (212 kDa) and ensure adequate protein transfer; cross-reactivity with mouse and rat should be confirmed in your specific experimental system.
• Given the immunogen design (amino acids 900-1000), the antibody may recognize intracellular epitopes; permeabilization may be required for immunofluorescence.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, including validated antibodies for targets like CACNA1S in skeletal muscle biology and excitation-contraction coupling studies. We offer flexible sourcing of polyclonal and monoclonal antibodies, bulk ancillary reagents, and tailored procurement for rare target raw materials. Connect with CamelBio to streamline your assay development from concept to clinic.