Anti-CACNA1H Polyclonal Antibody for IF/ICC, ELISA - O95180

Core Product Specifications and Parameters

Biological Background: CACNA1H Function and Localization

• CACNA1H encodes Cav3.2, a voltage-sensitive T-type calcium channel that opens at relatively negative potentials and exhibits voltage-dependent inactivation (PubMed:27149520, PubMed:9670923, PubMed:9930755). Related references: PMID:27149520 PMID:9670923 PMID:9930755
• Cav3.2 is implicated in pacemaking functions in neurons and cardiac cells, and may modulate neuronal firing patterns (PubMed:15048902). Related references: PMID:15048902
• In the adrenal zona glomerulosa, CACNA1H participates in aldosterone production in response to angiotensin II or hyperkalemia (PubMed:25907736, PubMed:27729216). Related references: PMID:25907736 PMID:27729216
• CACNA1H is expressed in the adrenal glomerulosa (protein level), kidney, liver, heart (including blood vessels), and brain regions such as amygdala, caudate nucleus, and putamen (PubMed:25907736, PubMed:27729216, PubMed:9670923, PubMed:9930755). Related references: PMID:25907736 PMID:27729216 PMID:9670923 PMID:9930755
• In testis, CACNA1H is primarily expressed in germ cells (PubMed:11751928). Related references: PMID:11751928
• The Cav3.2 protein is a glycoprotein with multiple transmembrane helices, contains zinc-binding sites, undergoes alternative splicing, and disease-associated mutations are linked to epilepsy.
• The full-length human CACNA1H protein has a calculated molecular weight of approximately 259 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal fragment (amino acids 1890–2030); confirm that the antibody detects full-length and relevant splice variants in your sample system.
• For immunofluorescence (IF/ICC), optimize fixation and permeabilization protocols, as Cav3.2 is a multi-pass transmembrane protein.
• In ELISA applications, use recombinant CACNA1H protein or the immunogen peptide as a positive control and include appropriate blocking steps to minimize background.
• Cross-reactivity with mouse CACNA1H is reported; validate antibody specificity in mouse tissues if required for your study.
• As a polyclonal antibody, verify lot-to-lot consistency for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw material partner, providing diagnostic manufacturers, labs, and research institutes with comprehensive access to validated antibodies, bulk reagents, and rare-target sourcing. This anti-CACNA1H polyclonal antibody supports research into ion channel biology, neurological disorders, and cardiovascular physiology. We offer one-stop procurement for antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents to streamline your assay development from concept to clinic.