Anti-CACNA1D Rabbit Polyclonal Antibody for WB and ELISA - Q01668

Core Product Specifications and Parameters

Biological Background: CACNA1D Function and Localization

• CACNA1D encodes the alpha-1D subunit (Cav1.3), a pore-forming component of voltage-dependent L-type calcium channels.
• Mediates L-type calcium currents critical for muscle contraction, hormone/neurotransmitter secretion, gene expression, and cell excitability; sensitive to dihydropyridine blockers.
• Belongs to the high-voltage activated (HVA) calcium channel family; alternative splicing generates multiple isoforms with distinct functional properties.
• Localizes to the plasma membrane, serving as an integral membrane protein in excitable cells.
• Robustly expressed in pancreatic islets (involved in insulin secretion) and brain regions such as cerebral cortex, hippocampus, and basal ganglia; absent in skeletal muscle.
• Undergoes post-translational modifications including glycosylation and phosphorylation, which regulate channel trafficking and activity.
• Mutations in CACNA1D are associated with deafness, epilepsy, and other neurodevelopmental disorders (disease variants per UniProt).

Experimental Guidance and Technical Tips

• The immunogen targets the C-terminal intracellular region (amino acids 1882-2181), suitable for detecting total CACNA1D protein.
• For Western blot, expect a band near 245 kDa; use low-percentage SDS-PAGE gels and extended transfer times to resolve high-molecular-weight proteins.
• Confirm antibody specificity by testing in CACNA1D-expressing tissues or cell lines (e.g., brain, pancreatic islets); include appropriate negative controls.
• When performing ELISA, coat with recombinant CACNA1D protein or tissue/cell lysates and validate linearity and sensitivity.
• This antibody cross-reacts with rat; verify cross-reactivity in other species if required for your experimental model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your IVD development by providing validated polyclonal antibodies like this anti-CACNA1D (Cav1.3) for studying L-type calcium channels. With a comprehensive portfolio of monoclonal/polyclonal antibodies, recombinant proteins, and ancillary reagents, we support diagnostic manufacturers from concept to clinic. Reach out for bulk orders or custom raw-material sourcing tailored to your assay needs.