Anti-CABP7 Polyclonal Antibody for WB, IHC-P, ELISA - Q86V35

Core Product Specifications and Parameters

Biological Background: CABP7 Function and Localization

• Calcium-binding protein 7 (CABP7), also called Calneuron II or Calneuron-2, is encoded by the CABP7 (CALN2) gene in humans.
• It functions as a negative regulator of Golgi-to-plasma membrane trafficking by interacting with phosphatidylinositol 4-kinase beta (PI4KB) and inhibiting its activity, thereby modulating membrane protein transport.
• CABP7 belongs to the calneuron family of calcium-binding proteins and shares structural features with other EF-hand-containing proteins, although its primary role is in membrane traffic rather than calcium signaling per se.
• The protein localizes predominantly to the Golgi apparatus, trans‑Golgi network membrane, and perinuclear cytoplasm, and has also been detected at the cell membrane.
• It contains calcium‑binding domains, at least one transmembrane helix, and likely undergoes regulated membrane association; classified as a metal‑binding transmembrane protein.
• The UniProt record lists additional keywords: 3D‑structure, Calcium, Cell membrane, Cytoplasm, Golgi apparatus, Membrane, Metal‑binding, Reference proteome, Repeat, Transmembrane, Transmembrane helix, highlighting its multi‑domain architecture.
• The calculated molecular weight is approximately 24.5 kDa, consistent with the observed band in Western blotting.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a recombinant fragment spanning amino acids 100–180 of human CABP7. This region lies outside the predicted transmembrane segment and may provide access to the soluble domain, making it suitable for detecting both soluble and membrane‑associated pools of the protein.
• For Western blot (WB), a band of approximately 24 kDa is expected under reducing conditions; consider using appropriate molecular weight markers and validate the signal in the relevant sample system.
• In immunohistochemistry (IHC‑P), CABP7 signals are expected in the Golgi/perinuclear area; co‑staining with a Golgi marker may assist in confirming specificity. Antigen retrieval and antibody dilution should be optimized for the specific tissue.
• For ELISA, the antibody can be used for detection of recombinant or endogenous CABP7; plate coating and detection conditions need to be established empirically.
• The antibody cross‑reacts with mouse and rat CABP7 based on supplier data for the immunogen sequence. If using in these species, sequence alignment of the target region is recommended to confirm reactivity.
• Since CABP7 acts as a PI4KB interactor, functional studies may require careful control of Golgi integrity and membrane trafficking; antibody performance in experimental manipulations should be validated under the intended conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this anti‑CABP7 polyclonal antibody as part of a comprehensive portfolio for researchers studying membrane traffic and Golgi biology. We provide a one‑stop sourcing bridge for IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and even sourcing for rare‑target raw materials. Our supply chain supports diagnostic manufacturers and labs from early research through clinical development, helping you advance your projects efficiently.