Anti-CABP1 Polyclonal Antibody for WB, IHC-P, ELISA - Q9NZU7

Core Product Specifications and Parameters

Biological Background: CABP1 Function and Localization

• CABP1 (Calcium-binding protein 1), also known as calbrain or caldendrin, is a neuronal calcium sensor protein involved in sensory transduction, synapse function, and vision. The gene undergoes alternative splicing, producing multiple isoforms including the brain-specific calbrain.
• Modulates calcium-dependent activity of inositol 1,4,5-triphosphate receptors (ITPRs). Related references: PMID:14570872
• Inhibits agonist-induced intracellular calcium signaling. Related references: PMID:15980432
• Regulates voltage-gated calcium channels: enhances inactivation of P/Q-type channels and causes calcium-dependent facilitation while inhibiting inactivation of L-type channels by binding to CACNA1C. Related references: PMID:11865310, PMID:15140941
• Suppresses the calcium-dependent inactivation of CACNA1D.
• Inhibits TRPC5 channels. Related references: PMID:15895247
• Prevents NMDA receptor-induced cellular degeneration and is required for the normal transfer of light signals through the retina.
• Exhibits a broad subcellular distribution including cytoplasm, cytoskeleton, cell membrane, Golgi, and postsynaptic density; predominantly expressed in brain (hippocampus, habenular area, cerebellum) and retina, with calbrain isoform restricted to brain.

Experimental Guidance and Technical Tips

• The immunogen was a synthetic peptide spanning amino acids 1–100 of human CABP1 (accession NP_001028849.1). This N-terminal region is expected to be well-exposed in the native protein.
• For Western blotting, the predicted molecular weight is ~40 kDa. Due to alternative splicing and post-translational modifications, additional bands may be observed in some sample types.
• In immunohistochemistry (paraffin), CABP1 is known to be highly expressed in brain and retina; ensure appropriate positive control tissues such as hippocampus or cerebellum are included.
• For ELISA applications, consider using this polyclonal antibody as either capture or detection reagent, and validate performance with recombinant CABP1 protein.
• The antibody has been reported to cross-react with mouse and rat CABP1 based on sequence homology; experimental validation in the relevant species is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw material supplier serving diagnostic manufacturers, laboratories, and research institutes with a comprehensive, one-stop sourcing model from concept to clinic. This anti-CABP1 polyclonal antibody exemplifies our commitment to providing high-quality neurobiology and signal transduction reagents. Beyond individual antibodies, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare-target raw materials to accelerate your assay development and production.