Anti-CABLES1 Rabbit Polyclonal Antibody for WB and ELISA - Q8TDN4

Core Product Specifications and Parameters

Biological Background: CABLES1 Function and Localization

• CABLES1 (CDK5 and ABL1 enzyme substrate 1), also known as Ik3-1, is a cyclin-dependent kinase binding protein that plays a key role in cell cycle regulation and neuronal development.
• It enhances cyclin-dependent kinase tyrosine phosphorylation: it promotes CDK5 phosphorylation by ABL1, increasing CDK5 activity critical for neuronal development, and promotes CDK2 phosphorylation by WEE1, which decreases CDK2 activity and leads to growth inhibition.
• CABLES1 positively affects neuronal outgrowth, contributing to proper nervous system development.
• It acts as a regulator for p53/p73-induced cell death, implicating it in tumor suppression pathways.
• The protein localizes to both the nucleus and the cytoplasm, suggesting dynamic shuttling and diverse functional roles.
• At the protein level, CABLES1 is expressed in breast, pancreas, colon, and head and neck tissues; its expression is strongly decreased in more than half of cases of atypical endometrial hyperplasia and in more than 90% of endometrial cancers, indicating a potential tumor suppressor role.
• CABLES1 undergoes alternative splicing and is classified under keywords including phosphoprotein, cell cycle, and cell division.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody covers amino acids 1-368 of human CABLES1, representing nearly the full-length protein. This design may allow detection of total CABLES1 in various assays.
• For Western blot (WB), the predicted molecular weight is approximately 68 kDa (UniProt: 67.6 kDa). Standard denaturing and reducing conditions are recommended, with validation in relevant human or mouse cell lines.
• For ELISA, the polyclonal nature of the antibody can provide sensitive detection. Consider optimizing coating and detection conditions for your specific assay format.
• Cross-reactivity with human and mouse has been confirmed; consider testing in other species if needed.
• CABLES1 is expressed in tissues such as breast, pancreas, and colon, and is downregulated in certain cancers; include appropriate positive and negative controls when investigating expression changes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. For CABLES1 research in neuronal development, cell cycle, or cancer, we offer validated antibody pairs, optimized rabbit polyclonal antibodies like this one, bulk ancillary reagents, and rare target sourcing—helping you bridge from concept to clinic efficiently.