Anti-CA5B Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y2D0

Core Product Specifications and Parameters

Biological Background: CA5B Function and Localization

• CA5B (Carbonic anhydrase 5B, mitochondrial) is also known as carbonate dehydratase VB or CA-VB.
• It functions as a mitochondrial carbonic anhydrase, catalyzing the reversible hydration of carbon dioxide to bicarbonate and protons.
• This enzyme belongs to the alpha-carbonic anhydrase family and requires zinc as a cofactor for its lyase activity.
• CA5B is predominantly localized to the mitochondrion, consistent with its role in mitochondrial metabolism.
• Tissue expression analysis reveals strongest expression in heart, pancreas, kidney, placenta, lung, and skeletal muscle, but it is not expressed in liver.
• The protein contains a transit peptide for mitochondrial targeting and is part of the reference proteome, with a molecular weight of approximately 36 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human CA5B protein (aa 1-317). For Western blotting, a band at around 36 kDa is expected; validate in positive control samples such as heart or pancreas lysates.
• For immunofluorescence/ICC, anticipate mitochondrial staining. Counterstaining with a mitochondrial marker is recommended for co-localization confirmation.
• Cross-reactivity has been observed with mouse and rat, but signal intensity may vary; optimize antibody dilution for each species and application.
• As with any polyclonal antibody, lot-to-lot consistency should be verified, and inclusion of negative controls (e.g., CA5B knockout samples) is advised to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories streamlined access to IVD raw materials, including this CA5B rabbit polyclonal antibody, along with complementary reagents and technical support. Our portfolio encompasses validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing, helping bridge the gap from early-stage development to clinical application. For projects involving mitochondrial enzymes or carbonic anhydrases, CamelBio can assist in assembling complete assay systems and supply chain solutions.