Anti-CA5A Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P35218

Core Product Specifications and Parameters

Biological Background: CA5A Function and Localization

• CA5A (Carbonic anhydrase 5A), also known as carbonate dehydratase VA (CA-VA), is a mitochondrial enzyme belonging to the alpha-carbonic anhydrase family.
• Catalyzes the reversible hydration of carbon dioxide to bicarbonate, a zinc-dependent lyase reaction crucial for pH regulation and intermediary metabolism (PubMed:8356065, PubMed:24530203). Related references: PMID:8356065 PMID:24530203
• Provides bicarbonate (HCO3-) within the mitochondrial matrix, which is essential for the activity of key metabolic enzymes in the urea cycle and the Krebs cycle (PubMed:24530203). Related references: PMID:24530203
• Encoded by the CA5A gene (GeneID: 763) in humans; alternative splicing may yield multiple transcript variants.
• Localizes specifically to the mitochondrion, with import mediated by an N-terminal mitochondrial transit peptide.
• Features a catalytically essential zinc ion bound at the active site, characteristic of carbonic anhydrases.
• Mutations in CA5A are associated with mitochondrial disorders, including hyperammonemia, due to impaired urea cycle function.

Experimental Guidance and Technical Tips

• The immunogen (residues 39–305) corresponds to the mature CA5A protein after mitochondrial import and cleavage of the transit peptide, potentially providing specific detection of the processed form.
• For Western blotting, a band near 35 kDa is expected; optimize blocking and antibody dilution, and validate with positive control lysates from tissues with high CA5A expression (e.g., liver, kidney).
• In immunofluorescence/ICC, mitochondrial staining should be observed; co-staining with a mitochondrial marker is recommended to confirm localization.
• As a polyclonal antibody, lot-to-lot consistency may vary; titrate for each new application and consider using a consistent secondary antibody.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive procurement partner for IVD manufacturers, research labs, and diagnostic developers, providing one-stop access to high-quality raw materials, technical services, and consulting support from concept to clinic. This anti-CA5A rabbit polyclonal antibody is a valuable tool for investigating mitochondrial metabolism, urea cycle disorders, and carbonic anhydrase function in assay development. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing solutions for rare or challenging targets.