Anti-CA3 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P07451

Core Product Specifications and Parameters

Biological Background: CA3 Function and Localization

• Carbonic anhydrase 3 (CA3, also known as carbonate dehydratase III) is a member of the carbonic anhydrase family, which catalyzes the reversible hydration of carbon dioxide.
• CA3 is predominantly expressed in muscle tissue, indicating a muscle-specific physiological role.
• The enzyme localizes to the cytoplasm, consistent with its function in intracellular pH regulation and CO2 transport.
• Post-translational modifications of CA3 include acetylation, glutathionylation, and phosphorylation, which may modulate its activity or interactions.
• CA3 contains a zinc-binding active site typical of carbonic anhydrases, and it is classified as a lyase.
• The protein has a molecular weight of approximately 29.6 kDa, aligning with the observed 30 kDa band in western blotting.

Experimental Guidance and Technical Tips

• The immunogen spans full-length human CA3 (residues 1-260), likely generating antibodies against both linear and conformational epitopes.
• For Western blotting, expect a band at ~30 kDa. Use muscle tissue lysates as a positive control due to CA3's tissue specificity.
• In IHC-P, consider antigen retrieval and validate with muscle sections known to express CA3.
• For ELISA, the antibody can be applied in direct or sandwich formats; pair with a suitable detection antibody for optimal sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, providing this anti-CA3 polyclonal antibody for muscle biomarker and carbonic anhydrase research. With capabilities in supplying validated antibody pairs, recombinant proteins, and ancillary reagents, we support your diagnostic development from concept to clinic. Reach out to explore custom sourcing for rare or specialized targets to accelerate your assay workflow.