Anti-CA13 Polyclonal Antibody for WB, IHC-P, ELISA - Q8N1Q1

Core Product Specifications and Parameters

Biological Background: Carbonic Anhydrase 13 Function and Localization

• CA13 is a cytoplasmic carbonic anhydrase that catalyzes the reversible conversion of CO₂ to bicarbonate, essential for pH homeostasis.
• It belongs to the alpha-carbonic anhydrase family, requiring zinc as a cofactor for its lyase activity.
• Expressed in thymus, small intestine, spleen, prostate, ovary, colon, and testis, indicating tissue-specific roles.
• Subcellularly localized to the cytosol, consistent with its role in intracellular pH regulation.
• The protein has a molecular weight of ~29.4 kDa and its 3D structure has been determined (Uniprot Q8N1Q1).
• Identified as a reference proteome component and detected in proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen covers aa 1‑220 of human CA13; sequence alignment with mouse orthologs may explain the observed cross‑reactivity.
• For Western blot, expect a band near 29 kDa; validate using lysates from CA13‑expressing tissues (e.g., thymus, colon).
• For IHC‑P, optimize antigen retrieval and test on positive (e.g., ovary) and negative (e.g., liver) control tissues.
• ELISA protocols may use recombinant CA13 or tissue extracts; confirm linearity and sensitivity empirically.
• Polyclonal nature suggests potential lot‑to‑lot variability; include internal positive controls in each experiment.
• Cross‑reactivity with mouse enables translational studies, but verify specificity in genetically modified models if available.

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CamelBio connects diagnostic manufacturers and labs with a curated portfolio of IVD raw materials, exemplified by this anti‑CA13 polyclonal antibody for pH‑regulation and metabolic research. We can source validated antibody pairs, optimized monoclonals, bulk ancillary reagents, and rare‑target raw materials to support your IVD workflow. Let CamelBio be your sourcing bridge, simplifying procurement from concept to clinical application.