Anti-C9orf72 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q96LT7

Core Product Specifications and Parameters

Biological Background: C9orf72 Function and Localization

• C9orf72 (also known as DENND9, ALSFTD, FTDALS1) encodes a guanine nucleotide exchange factor that belongs to the DENN domain-containing protein family and exists as two widely expressed isoforms.
• Functions as a GEF for several Rab GTPases, including RAB39A, RAB8A, and RAB39B, facilitating autophagosome-lysosome tethering and fusion during autophagy. Related references: PMID:27103069, PMID:27193190, PMID:27617292, PMID:37821429
• The C9orf72-SMCR8 complex also exhibits GTPase-activating protein (GAP) activity toward RAB8A and RAB11A. Related references: PMID:32303654
• Regulates mTORC1 signaling by promoting phosphorylation of downstream substrates, linking nutrient sensing to autophagy. Related references: PMID:27559131
• Involved in endosomal trafficking and phagosome maturation, and modulates actin dynamics in motor neurons via ARF6 inactivation and cofilin activation, thereby regulating axon extension. Related references: PMID:24549040, PMID:27723745
• Subcellularly localizes to multiple compartments, including cytoplasm, nucleus, endosomes, lysosomes, autophagosomes, axons, growth cones, and synaptic sites, reflecting its diverse cellular roles.
• Widely expressed in tissues, with notable presence in kidney, lung, liver, brain (cerebellum, frontal cortex), and immune cells (lymphoblasts).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 9-200) of human C9orf72 (isoform NP_001242983.1); the antibody may recognize both full-length and truncated isoforms.
• For Western blotting, a band around 54 kDa is expected in human, mouse, and rat lysates; consider using positive control cell lines or tissues known to express C9orf72.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize antigen retrieval conditions and include isotype controls to confirm specificity.
• For ELISA, the recombinant immunogen or cell lysates can serve as positive controls; consider cross-reactivity testing with closely related DENN domain proteins if needed.
• As with any polyclonal antibody, lot-to-lot consistency should be verified for critical quantitative applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to premium IVD raw materials, including validated polyclonal and monoclonal antibodies. This anti-C9orf72 polyclonal antibody supports studies in autophagy, neurodegeneration, and amyotrophic lateral sclerosis (ALS), offering a reliable tool for applications like WB, IHC-P, IF/ICC, and ELISA. If your project demands customized antibody pairs, bulk ancillary reagents, or rare target raw-material sourcing, CamelBio’s integrated supply chain and technical expertise can bridge the gap from concept to clinic.