Anti-C9orf114 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q5T280

Core Product Specifications and Parameters

Biological Background: C9orf114 Function and Localization

• C9orf114 (also known as CENP32, SPOUT1) is a 28S rRNA uridine-N(3)-methyltransferase that catalyzes S-adenosyl-L-methionine-dependent methylation of uridine in 28S rRNA, a modification critical for ribosome function. Related references: PMID:39671501 PMID:39962046

• It plays an essential role in mitotic spindle organization by ensuring proper association of centrosomes with the poles of the bipolar spindle during metaphase, thereby contributing to accurate chromosome alignment. Related references: PMID:20813266 PMID:25657325 PMID:39962046

• The protein acts as a kinetochore-associated factor and localizes to centromeres and centrosomes, bridging centromeric and centrosomal functions during cell division.

• C9orf114 may promote centrosome maturation by recruiting A-kinase anchor protein AKAP9 to centrosomes in early mitosis, facilitating proper spindle assembly. Related references: PMID:25657325

• Beyond its mitotic role, C9orf114 binds specifically to the MIR145 hairpin precursor and post-transcriptionally regulates MIR145 expression, linking its function to microRNA processing. Related references: PMID:28431233

• The protein is a member of the SPOUT domain-containing methyltransferase superfamily and carries a characteristic S-adenosylmethionine-binding fold.

• Mutations in the SPOUT1 gene have been associated with neurological disorders including epilepsy and intellectual disability, underscoring its importance in human development.

Experimental Guidance and Technical Tips

• The immunogen encompasses the central region (aa 163–340) of human C9orf114, likely recognizing multiple epitopes; consider this when designing experiments that require precise domain recognition.
• For Western blot, load 20–40 µg of total protein lysate from human or rat cell lines and probe; the expected molecular weight is approximately 42 kDa. Confirm band specificity by comparing with a negative control or by siRNA knockdown.
• In IHC-P, perform antigen retrieval and validate staining in tissue sections known to express C9orf114 (e.g., proliferative tissues with active mitosis). Observe centromeric and centrosomal localization in mitotic cells.
• For ELISA, the antibody can be used as a capture or detection reagent; optimize coating concentration and detection conditions empirically.
• As a polyclonal antibody, be aware of potential lot-to-lot variability and re-validate when using a new batch.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and research communities with a comprehensive portfolio of IVD raw materials and technical services. This anti-C9orf114 antibody is part of our extensive cell biology antibody collection, which includes validated clones for critical mitosis and gene expression targets. In addition to ready-to-use reagents, we offer custom antibody development, bulk ordering, and one-stop supply of ancillary reagents to streamline your assay development from concept to clinic.