Anti-C7orf50/Cholesin Polyclonal Antibody for WB, ELISA - Q9BRJ6

Core Product Specifications and Parameters

Biological Background: C7orf50/Cholesin Function and Localization

• C7orf50 (chromosome 7 open reading frame 50) encodes protein cholesin, a secreted hormone that plays a key role in cholesterol homeostasis.
• Cholesin is released from intestinal epithelial cells upon dietary cholesterol absorption and acts on the liver to suppress cholesterol biosynthesis and VLDL secretion, ultimately lowering circulating cholesterol levels.
• This hormone signals through the G protein-coupled receptor GPR146, linking gut cholesterol sensing to hepatic metabolic responses.
• As a secreted protein, cholesin is localized extracellularly and circulates in blood to reach target tissues.
• Expression is predominantly intestinal, and secretion is acutely induced by feeding and cholesterol uptake, highlighting its nutrient-responsive regulation.
• UniProt annotates cholesin as a phosphoprotein (keyword: Phosphoprotein), suggesting post-translational modification may modulate its activity.
• The protein has a predicted molecular weight of approximately 22 kDa.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to the full-length 1–194 amino acid sequence of human C7orf50, providing comprehensive epitope coverage for polyclonal antibody generation.
• Recommended applications include Western blot (WB) and ELISA, as validated by the supplier; for other applications, consider pilot testing under your experimental conditions.
• When performing WB, expect a band around 22 kDa; validate the antibody in relevant tissue lysates such as intestinal or liver samples, and include appropriate controls.
• Given cross-reactivity with mouse, this antibody is suitable for both human and mouse studies; verify species-specific reactivity if using other models.
• For ELISA, consider paired antibody panels or sandwich formats if quantitating cholesin levels in serum or conditioned media.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop sourcing bridge for IVD raw materials, offering validated polyclonal and monoclonal antibodies, optimized antibody pairs, and bulk ancillary reagents tailored to cholesterol metabolism and hormone research. Our portfolio includes rare-target antibodies like anti-cholesin, ensuring diagnostic manufacturers and labs access critical tools for assay development from concept to clinic. Whether scaling up for production or seeking technical consultation, CamelBio streamlines your supply chain with quality-assured materials.