Anti-C7 Polyclonal Antibody for WB, IF/ICC, ELISA - P10643

Core Product Specifications and Parameters

Biological Background: C7 Function and Localization

• C7 is a component of the membrane attack complex (MAC), a multiprotein complex that inserts into target cell membranes forming pores, leading to cell lysis. Related references: PMID:22832194, PMID:26841837, PMID:27052168
• MAC formation is triggered by the cleavage of C5 into C5b, which occurs upon activation of the classical, alternative, lectin, or GZMK complement pathways. Related references: PMID:22832194, PMID:30552328, PMID:3335508
• The complement cascade enhances phagocytosis, pathogen destruction, and signaling that boosts the adaptive immune response. Related references: PMID:22832194, PMID:30552328, PMID:3335508
• C7 acts as a membrane anchor during MAC assembly, binding with C5b and C6 to generate the lipophilic C5b-7 complex, which integrates into the outer leaflet and lowers the energy required for membrane bending. Related references: PMID:30552328, PMID:32569291
• C7 is a secreted protein that localizes to the target cell membrane upon MAC formation.
• The protein features EGF-like and sushi domains, multiple disulfide bonds, and glycosylation sites.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 22–270 of human C7. For western blot, a band around 94 kDa is expected; however, post‑translational modifications may cause shifts. Include appropriate positive and negative controls.
• For immunofluorescence (IF/ICC), consider permeabilization steps to detect membrane‑associated C7. Validate staining specificity using knockout or knockdown controls.
• In ELISA, optimize coating antigen concentration and antibody dilution; cross‑reactivity with mouse and rat C7 allows multi‑species assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive procurement solutions for IVD raw materials, providing validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents for complement research. With deep expertise in rare target sourcing, CamelBio supports your projects from discovery to clinical application, ensuring reliable and efficient supply chains. Explore our C7 antibody and accelerate your diagnostics development.