Anti-C6orf25 (G6b) Rabbit Polyclonal Antibody for WB and ELISA - O95866

Core Product Specifications and Parameters

Biological Background: G6b Function and Localization

• Encoded by the MPIG6B gene, G6b (also known as C6orf25, G6b-B, NG31, THAMY) is a megakaryocyte and platelet inhibitory receptor that belongs to the immunoglobulin superfamily.
• Acts as a critical regulator of hematopoietic lineage differentiation, megakaryocyte function, and platelet production by inhibiting platelet aggregation and activation in response to agonists such as ADP and collagen-related peptide (PubMed:12665801). Related references: PMID:12665801
• Contains two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in its cytoplasmic tail, through which it inhibits CLEC1B and GP6:FcRgamma signaling pathways to regulate platelet function (PubMed:17311996, PubMed:27743390). Related references: PMID:17311996, PMID:27743390
• Isoform B is the only isoform containing both a transmembrane region and two ITIMs; isoform A may act as an activating counterpart.
• Subcellularly localizes to the endoplasmic reticulum, Golgi apparatus, and cell membrane.
• Expressed primarily in platelets and restricted hematopoietic cell lines (K-562, MOLT-4, Jurkat), but not detected in monocyte, B-cell, fibroblast, or natural killer cell lines.
• Post-translationally modified by phosphorylation (phosphoprotein) and contains a heparin-binding domain.
• Keywords highlight its roles as a glycoprotein, heparin-binding protein, and transmembrane signaling receptor.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 18–142) of human C6orf25, which is conserved across isoforms and species; this may enable detection of both isoform A and B in human, mouse, and rat samples.
• For western blotting, a predominant band around 26 kDa is expected in human platelet or megakaryocyte lysates; validate the exact migration pattern in your experimental system.
• Cross-reactivity with mouse and rat allows for potential use in rodent models; however, verify specificity in the relevant tissue or cell line.
• In ELISA applications, this polyclonal antibody can be used as a capture or detection reagent; consider optimizing coating concentration and pairing with a specific monoclonal antibody for sandwich ELISA formats.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For researchers focused on platelet biology and megakaryocyte inhibitory receptor pathways, this anti-C6orf25 antibody is part of our comprehensive portfolio of validated reagents. Our supply chain supports bulk orders, custom antibody development, and ancillary reagents to accelerate your diagnostic assay development and biomarker studies.