Anti-C5/C5a Polyclonal Antibody for WB, ELISA - P01031

Core Product Specifications and Parameters

Biological Background: Complement C5 Function and Localization

• Complement C5 (C5) is a central component of the complement system, serving as the precursor for the anaphylatoxin C5a and the membrane attack complex (MAC) component C5b. Related references: PMID:12878586, PMID:18204047, PMID:30643019
• Upon cleavage by C5 convertase, C5b initiates MAC assembly by sequentially binding C6, C7, C8, and multiple C9 molecules, forming a pore that lyses target cells. Related references: PMID:26841837, PMID:27052168, PMID:30552328, PMID:30643019, PMID:32569291
• The C5a fragment acts as a potent inflammatory mediator by binding to receptors C5AR1 and C5AR2, triggering chemotaxis, histamine release, and smooth muscle contraction. Related references: PMID:8182049, PMID:9553099, PMID:342601, PMID:37852260, PMID:37169960, PMID:36806352
• C5 is a secreted glycoprotein that associates with the target cell membrane during MAC formation, emphasizing its role in both fluid-phase and membrane-bound immunity.
• The protein belongs to the alpha-2-macroglobulin family and contains a C3/PZP-like domain, with structural features including intra-chain disulfide bonds and multiple N-glycosylation sites.
• Disease variants in C5 are linked to complement deficiencies and susceptibility to recurrent infections.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1567–1676, corresponding to a C-terminal region of human C5 that is likely present in the C5b fragment; consider this when interpreting results for C5a versus C5b detection.
• For Western blot (WB), the full-length C5 protein has a predicted molecular weight of approximately 188 kDa; cleavage products may also be observed depending on sample preparation.
• In ELISA applications, validation with appropriate recombinant proteins (e.g., C5a or C5b) is recommended to confirm specificity.
• The reported cross-reactivity with mouse and rat suggests this antibody may be useful for translational studies in rodent models; however, verify specificity in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner, connecting diagnostic manufacturers and research laboratories with verified antibody reagents and technical support. This anti-C5/C5a polyclonal antibody supports complement system and inflammation research, and fits into a broader portfolio that includes validated antibody pairs, optimized monoclonals, and ancillary bulk reagents. For rare or custom-target raw materials, CamelBio’s integrated supply network can streamline procurement from concept to clinical application.