Anti-C5AR2 Rabbit Polyclonal Antibody for WB, ELISA - Q9P296

Core Product Specifications and Parameters

Biological Background: C5AR2 Function and Localization

• C5AR2, also known as C5L2, GPF77, and GPR77, is a G protein-coupled receptor (GPCR) that binds anaphylatoxins C5a and their dearginated forms (ASP/C3adesArg, C4adesArg, C5adesArg).
• Upon C5a binding, C5AR2 stimulates chemotaxis, granule enzyme release, intracellular calcium release, and superoxide anion production. Related references: PMID:11773063
• C5AR2 signals via G(i)/G(o) G alpha proteins (GNAI1 or GNAO1), leading to inhibition of adenylate cyclase. Related references: PMID:12540846
• The receptor also recognizes dearginated peptides ASP/C3adesArg, C4adesArg, and C5adesArg, with ligand binding triggering a conformational change modulating downstream effectors. Related references: PMID:12540846 PMID:15833747 PMID:19615750
• Subcellularly, C5AR2 is a multi-pass membrane protein that localizes to the cell membrane.
• Tissue expression includes frontal cortex, hippocampus, hypothalamus, pons, and liver.
• The protein features disulfide bonds, glycosylation, and phosphorylation sites as annotated in UniProt.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 258-337) of human C5AR2, suggesting specific recognition of the receptor's intracellular domain.
• For Western blot (WB), the predicted molecular weight is 36 kDa; however, due to post-translational modifications such as glycosylation and phosphorylation, the observed band may migrate at a higher apparent molecular weight. Validate using appropriate positive controls (e.g., C5AR2-expressing cell lines).
• In ELISA applications, the antibody can be employed for both direct and indirect detection of the C5AR2 protein or its fragments. Optimize coating and detection conditions for best signal-to-noise ratio.
• The antibody shows cross-reactivity with both human and mouse C5AR2, making it suitable for translational studies. Always verify specificity in your experimental system.
• Consider using this polyclonal antibody in conjunction with C5AR2 agonists (e.g., C5a) or antagonists to study receptor activation and downstream signaling.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated C5AR2 antibodies for inflammation and immunology studies. We offer optimized polyclonal antibodies, matched antibody pairs, and bulk ancillary reagents to support your assay development. Our sourcing expertise ensures timely delivery of even rare-target materials, helping you advance from concept to clinic efficiently.