Anti-C5AR1 Polyclonal Antibody for WB, IHC-P, ELISA - P21730

Core Product Specifications and Parameters

Biological Background: C5AR1 Function and Localization

• Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a, stimulating chemotaxis, granule enzyme release, intracellular calcium release, and superoxide anion production. Related references: PMID:10636859, PMID:15153520, PMID:1847994
• Signaling is mediated via guanine nucleotide-binding proteins (G proteins), specifically coupled to G(i)/G(o) alpha subunits (GNAI1, GNAO1), inhibiting adenylate cyclase. Related references: PMID:36806352, PMID:37852260
• Ligand binding involves a two-site model: a high-affinity site on the extracellular N-terminus and an activation site in the transmembrane region that triggers downstream signaling. Related references: PMID:7622471, PMID:8182049, PMID:9553099
• Also known as C5a-R, C5aR, CD88, or C5R1; a member of the G protein-coupled receptor superfamily.
• Predominantly located on the cell membrane and in cytoplasmic vesicles.
• Post-translational modifications include glycosylation, phosphorylation, sulfation, and disulfide bond formation.
• Key mediator of immune cell chemotaxis and inflammatory responses.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 1-282) encompasses the extracellular N-terminus and part of the transmembrane domains; consider its suitability for detecting both surface and total C5AR1 in native and denaturing conditions.
• For Western blot, the predicted molecular weight is approximately 39 kDa; however, glycosylation may cause the observed band to shift higher. Validate in relevant sample systems.
• Optimize antibody dilution for IHC-P using known positive tissues (e.g., immune cell-rich tissues) and standard antigen retrieval methods.
• In ELISA, this antibody may be used as a capture or detection reagent; confirm cross-reactivity with mouse and rat samples experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop supplier of IVD raw materials, supporting diagnostic developers from concept to clinic. Our portfolio includes validated antibodies targeting complement receptors like C5AR1, along with bulk ancillary reagents and customized sourcing for rare targets. Partner with us for reliable antibody pairs, optimized polyclonal/monoclonal antibodies, and comprehensive technical support to streamline your assay development.