Anti-C4BPB Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P20851

Core Product Specifications and Parameters

Biological Background: C4BPB Function and Localization

• C4BPB, also known as C4b-binding protein beta chain, is a key regulator of the classical complement pathway. It functions as a cofactor for C3b/C4b inactivator (C3bINA) to hydrolyze C4b, and accelerates the decay of the C3 convertase (C4bC2a) by dissociating C2a.
• The protein is secreted and primarily circulates in the plasma as part of the C4b-binding protein complex, modulating innate immune responses.
• C4BPB contains multiple Sushi (CCP/SCR) domains, characteristic of complement regulatory proteins, and is known to bind protein S, an anticoagulant factor, linking complement and coagulation pathways.
• Post-translational modifications include glycosylation and disulfide bond formation, which are essential for its structural stability and function.
• The gene C4BPB undergoes alternative splicing, generating isoforms that may have distinct regulatory roles.
• It is classified under immunity, innate immunity, and complement pathway keywords, highlighting its biological significance.

Experimental Guidance and Technical Tips

• For Western blot, use liver or plasma samples where C4BPB is abundantly expressed. Include a positive control and consider loading controls appropriate for secreted proteins.
• For ELISA, the antibody can be used for detection or capture, but optimal dilution should be determined experimentally.
• For immunofluorescence, ensure proper fixation and permeabilization; antigen retrieval may enhance signal in paraffin-embedded sections.
• As the immunogen covers a large portion of the protein (aa 17-251), the antibody may recognize multiple isoforms and degradation products; validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with streamlined access to high-quality IVD raw materials. This C4BPB antibody supports complement pathway studies and assay development. Beyond antibodies, we offer one-stop sourcing for recombinant proteins, ancillary reagents, and customized raw material solutions to accelerate your diagnostic projects from concept to clinic.