Anti-C3c Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P01024

Core Product Specifications and Parameters

Biological Background: Complement C3 Function and Localization

• Complement C3 is a precursor protein that serves as a central component of the innate immune system, participating in the classical, alternative, and lectin complement pathways. It is cleaved to generate C3a and C3b fragments that mediate opsonization, inflammation, and pathogen clearance. Related references: PMID:28264884, PMID:31507604, PMID:3653927
• Following cleavage by C3 convertase, C3b attaches to microbial surfaces via a reactive thioester bond, serving as an opsonin. Together with complement C4b, it recruits C2b to form the classical C5 convertase that activates C5. In the alternative pathway, C3b recruits factor B (CFB) to form the alternative C5 convertase. Related references: PMID:28264884, PMID:31507604, PMID:3653927, PMID:6903192, PMID:12878586, PMID:18204047, PMID:2387864, PMID:624565, PMID:6554279
• C3a, a small anaphylatoxin released during complement activation, binds to its receptor C3AR1 and promotes inflammatory responses. It stimulates mast cells and basophils to release histamine and cytokines, and acts as a potent chemoattractant for macrophages and neutrophils. Related references: PMID:6968751, PMID:37169960, PMID:37852260, PMID:8702752, PMID:23383423, PMID:342601, PMID:5778786
• In adipose tissue, C3a acts as an adipogenic hormone by stimulating triglyceride synthesis and glucose transport via the C5AR2 receptor, without pro-inflammatory activity. This contributes to postprandial lipid clearance and fat storage. Related references: PMID:10432298, PMID:15833747, PMID:16333141, PMID:11773063, PMID:12540846, PMID:19615750, PMID:37852260
• C3 and its fragments are secreted proteins found in plasma, but they can also attach to cell surfaces. C3 is produced in the liver and by adipocytes.
• Mutations in the C3 gene are associated with age‑related macular degeneration and atypical hemolytic uremic syndrome (aHUS). The protein undergoes extensive post-translational modifications including glycosylation, disulfide bond formation, and a critical thioester bond that enables covalent attachment to target surfaces.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C‑terminal fragment (aa 1364–1663) of human C3c; the resulting polyclonal antibody may recognize C3c, C3b, and full‑length C3.
• For Western blotting, a band at approximately 187 kDa is expected under reducing conditions; additional bands corresponding to cleavage fragments may be observed.
• In immunofluorescence (IF‑P), the antibody may detect cell surface‑bound or secreted C3 in fixed cells; appropriate permeabilization and controls should be included.
• Consider validating the antibody in your specific sample system (e.g., plasma, serum, or cell lysates) and confirm reactivity with recombinant C3c if cross‑reactivity with other complement proteins is a concern.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories streamlined access to high‑quality IVD raw materials. For complement pathway targets such as C3/c3c, we supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents through a reliable sourcing network. Our expertise ensures consistent supply for your immunoassay and research development needs.