Anti-C2CD5 Rabbit Polyclonal Antibody for WB and ELISA - Q86YS7

Core Product Specifications and Parameters

Biological Background: C2CD5 Function and Localization

• C2CD5 (C2 domain-containing protein 5), also known as CDP138 or KIAA0528, is a 138 kDa phosphoprotein containing C2 domains that mediate calcium-dependent phospholipid binding.
• C2CD5 plays a critical role in insulin-stimulated glucose transport by facilitating the translocation of glucose transporter GLUT4 from intracellular storage vesicles to the plasma membrane in adipocytes.
• The protein is essential for optimal membrane fusion between GLUT4 storage vesicles and the plasma membrane, ensuring efficient glucose uptake upon insulin signaling.
• C2CD5 localizes to cytoplasmic vesicle membranes, the cell cortex, cell membrane, and ruffles, consistent with its role in vesicle trafficking and membrane dynamics.
• It binds phospholipid membranes in a calcium-dependent manner, a typical property of C2 domain proteins, and is involved in protein transport pathways.
• Post-translational modifications include phosphorylation (as indicated by its alias CDP138 and phosphoprotein keyword), suggesting regulation by insulin signaling cascades.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 732-1051) of human C2CD5, which may produce antibodies recognizing multiple isoforms due to alternative splicing.
• For western blotting, consider using whole cell lysates from insulin-responsive cells (e.g., 3T3-L1 adipocytes or L6 myotubes) and validate band specificity with molecular weight markers around 110 kDa.
• ELISA applications may be optimized using recombinant C2CD5 protein as a coating antigen; cross-reactivity with mouse and rat orthologs suggests utility in rodent models.
• As a polyclonal antibody, lot-to-lot consistency may vary; we recommend validating each new batch in your specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and research communities with a comprehensive portfolio of IVD raw materials, including validated antibodies for metabolic and signaling targets like C2CD5. Beyond this polyclonal antibody, we offer monoclonal antibody development, bulk ancillary reagents, and customized rare-target sourcing to streamline your assay development from concept to clinic.