Anti-C1R Rabbit Polyclonal Antibody for IHC-P, IF/ICC, ELISA - P00736

Core Product Specifications and Parameters

Biological Background: Complement C1r subcomponent Function and Localization

• C1R is the serine protease component of the C1 complex, which initiates the classical complement pathway upon recognition of antibody-antigen complexes.
  Related references: PMID:17996945, PMID:19473974, PMID:29449492
• Activation of C1R occurs via autocatalytic cleavage triggered by C1Q binding to immunoglobulins.
  Related references: PMID:11445589, PMID:11673533, PMID:17996945
• Activated C1R cleaves and activates C1S, the second protease of the classical pathway, leading to downstream complement activation.
  Related references: PMID:11445589, PMID:11673533, PMID:6271784
• C1R is a secreted protein but also localizes to the cell surface.
• Post-translational modifications include glycosylation, phosphorylation, and hydroxylation, as indicated by proteomics analyses.
• Mutations in the C1R gene are associated with Ehlers-Danlos syndrome type 8 (EDS8), characterized by periodontal fragility.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a recombinant fragment covering amino acids 18-190 of human C1R, targeting an N-terminal region that may encompass domains involved in protein-protein interactions; this design may favor antibodies recognizing the non-catalytic portion of the protein.
• For IHC-P applications, consider optimizing antigen retrieval and antibody dilution using a relevant tissue panel; note that C1R is secreted and may require gentle fixation to preserve extracellular localization.
• For IF/ICC, permeabilization and blocking steps should be optimized for the cell type of interest; validate staining specificity with appropriate controls (e.g., C1R knockout or siRNA knockdown if available).
• In ELISA, this antibody can be employed as a detection or capture reagent; due to its polyclonal nature, assess lot-to-lot consistency and cross-reactivity in the relevant sample matrix.
• Always confirm the antibody's performance in the intended application with positive and negative controls, as cross-reactivity with mouse and rat C1R has been observed but may vary across assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with comprehensive access to IVD raw materials, including validated antibody reagents like this C1R polyclonal antibody. For complement system research and autoimmune disease diagnostics, we offer polyclonal and monoclonal antibodies, matched antibody pairs, and ancillary reagents in bulk quantities. Our sourcing services extend to rare targets, ensuring a reliable supply chain for your assay development needs.