Anti-C1R Polyclonal Antibody for IF/ICC and ELISA - P00736

Core Product Specifications and Parameters

Biological Background: C1R Function and Localization

• C1R, also known as Complement C1r subcomponent, is a serine protease that is part of the C1 complex, which initiates the classical complement pathway, leading to pathogen phagocytosis and immune signaling. Related references: PMID:17996945 PMID:19473974 PMID:29449492
• Upon binding of the C1q subcomplex to antigen-antibody complexes on pathogen surfaces, C1R undergoes autocatalytic cleavage and activation. Related references: PMID:11445589 PMID:11673533 PMID:17996945
• Activated C1R cleaves and activates C1S, the second serine protease in the classical complement cascade. Related references: PMID:11445589 PMID:11673533 PMID:6271784
• C1R is a secreted protein found in blood and can also localize to the cell surface.
• The protein features Sushi repeats, an EGF-like domain, and a serine protease domain, and is modified by glycosylation, disulfide bond formation, and hydroxylation.
• Mutations in the C1R gene are associated with Ehlers-Danlos syndrome type VIII (EDS8), a connective tissue disorder.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 466–705) encompassing the serine protease domain; this design may favor detection of the activated form or full-length protein.
• For IF/ICC, consider appropriate permeabilization and validate in complement-relevant cell lines or tissues to confirm specificity.
• In ELISA applications, the antibody may be used for capture or detection of C1R in biological fluids; optimize conditions for protein stability and assay sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw-material sourcing partner, providing high-quality reagents like this anti-C1R polyclonal antibody for complement and immune research. Beyond single antibodies, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw materials to streamline your assay development from concept to clinic.