Anti-C1QTNF5 Polyclonal Antibody for WB and ELISA - Q9BXJ0

Core Product Specifications and Parameters

Biological Background: C1QTNF5 Function and Localization

• C1QTNF5, also known as CTRP5 or MFRP, is a secreted protein belonging to the C1q/TNF-related protein (CTRP) family. It is encoded by the C1QTNF5 gene in humans.
• The protein contains an N-terminal signal peptide, a collagen-like domain (UniProt keyword: Collagen), and a C-terminal globular C1q domain typical of this family.
• UniProt annotations indicate it is secreted, and the protein is involved in extracellular matrix organization and cell signaling, with known disease variants associated with retinal degeneration (keyword: Disease variant).
• The molecular weight is approximately 25 kDa, consistent with the 25 kDa band observed in Western blotting under reducing conditions.
• The antibody was raised against a recombinant protein spanning amino acids 16-243, covering the majority of the mature protein, including the collagen and C1q domains, and is expected to recognize full-length native C1QTNF5.

Experimental Guidance and Technical Tips

• As a polyclonal antibody, it may recognize multiple epitopes, providing robust signal in WB. Optimize antibody dilution using known positive controls.
• For WB, a band around 25 kDa is expected in human, mouse, and rat samples. Use appropriate positive controls, such as recombinant protein or cell lysates known to express C1QTNF5.
• For ELISA, this antibody can be used for capture or detection, though validation in the specific assay system is recommended. Perform standard curves with known antigen concentrations.
• Since the immunogen is a recombinant fusion protein, cross-reactivity with other CTRP family members cannot be excluded. Validate specificity with knockout or siRNA-treated samples.
• The antibody is shipped on ice bags. Upon receipt, store at -20°C or as recommended. Avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing solution for IVD raw materials, offering validated antibodies, recombinant proteins, and ancillary reagents to diagnostic manufacturers and research institutes. The C1QTNF5 rabbit polyclonal antibody is well-suited for studies on retinal degenerative disorders, extracellular matrix biology, and complement-related pathways. Our reliable supply chain and technical support accelerate your assay development from concept to clinic.