Anti-C1QC Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P02747

Core Product Specifications and Parameters

Biological Background: C1QC Function and Localization

• C1QC is the C chain of complement C1q, a recognition subcomponent of the C1 complex that initiates the classical pathway of the complement system. Related references: PMID:12847249 PMID:19006321 PMID:24626930

• Together with C1QA and C1QB, C1QC binds the Fc domains of antigen-complexed IgG or IgM antibodies on pathogen surfaces. Related references: PMID:12847249 PMID:19006321 PMID:24626930

• Antibody binding activates proenzyme C1R, which cleaves C1S to trigger a proteolytic cascade, leading to opsonization and pathogen clearance. Related references: PMID:29449492

• The C1q subcomplex is optimally activated by hexameric IgG immune complexes or pentameric IgM, reflecting distinct structural requirements. Related references: PMID:19706439 PMID:24626930 PMID:29449492

• C1q also recognizes phosphatidylserine exposed on apoptotic cells, suggesting an additional role in programmed cell death clearance and complement activation. Related references: PMID:18250442

• C1QC is secreted and can localize to the cell surface; its collagen-like domain undergoes post-translational modifications such as hydroxylation, disulfide bonding, and glycosylation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa 50–150) of human C1QC, excluding the signal peptide. This region likely targets the collagen-like domain; verify reactivity with endogenous C1q in appropriate cell or tissue models.
• For Western blotting, a specific band near 26 kDa is expected. Include reducing conditions and load controls; consider using stimulated immune cells or serum samples to detect native C1QC.
• In IHC-P, optimize antigen retrieval and antibody dilution. Positive staining may be observed in tissues with immune complex deposits (e.g., kidney, synovium) or in liver where complement proteins are synthesized.
• For ELISA, this polyclonal antibody can serve as a capture or detection reagent for C1q complex quantification. Pairing with monoclonal antibodies against other C1q chains may improve specificity.
• Cross-reactivity is confirmed for human; test on other species if required, as the immunogen sequence may be conserved.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including validated antibodies for the complement system and innate immunity. Whether you need bulk quantities of this anti-C1QC antibody, complementary antibody pairs for C1q detection, or customized sourcing for rare targets, our team offers reliable supply solutions and technical consultation to accelerate your assay development from concept to clinic.