Anti-C1QB Polyclonal Antibody for WB, ELISA - P02746

Core Product Specifications and Parameters

Biological Background: C1QB Function and Localization

• C1QB is a core component of the complement C1 complex, a multiprotein complex that initiates the classical pathway of the complement system, leading to pathogen phagocytosis and adaptive immune signaling. Related references: PMID:12847249, PMID:19006321, PMID:24626930

• The C1Q subcomplex, comprising C1QA, C1QB, and C1QC, specifically recognizes and binds IgG or IgM immunoglobulins complexed with antigens on pathogen surfaces. Related references: PMID:12847249, PMID:19006321, PMID:24626930

• Immunoglobulin binding activates proenzyme C1R, which cleaves C1S, initiating the proteolytic complement cascade. Related references: PMID:29449492

• The C1Q subcomplex can also recognize phosphatidylserine on apoptotic cells, potentially promoting complement system activation and clearance. Related references: PMID:18250442

• The C1Q subcomplex is activated by hexameric IgG or pentameric IgM immune complexes. Related references: PMID:19706439, PMID:24626930, PMID:29449492

• Subcellularly, C1QB is found secreted and at the cell surface.

• C1QB contains collagen-like regions, is glycosylated, and forms disulfide bonds, contributing to its structural stability and function.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 104-253 of human C1QB) includes the globular C1q domain, which may help reduce cross-reactivity with other C1q subunits when tested against full-length proteins.
• For western blotting, consider using reduced and non-reduced conditions, as C1QB contains disulfide bonds; a band near 27 kDa is expected under reducing conditions.
• In ELISA, the antibody may be suitable for capture or detection of recombinant or serum C1QB; titrate antibody and use appropriate blocking buffers.
• Validate antibody specificity using knockout cell lines or siRNA knockdown controls in human or mouse cells to confirm target recognition.
• Due to polyclonal nature, lot-to-lot variability could occur; include appropriate positive and negative controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research institutes, and laboratories with one-stop access to IVD raw materials and technical support from concept to clinic. This C1QB antibody supports complement system research, autoimmune disease studies, and pathogen-host interaction analysis. Our supply capabilities include validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets, ensuring efficiency in your diagnostic development workflows.