Anti-C1qa Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P98086

Core Product Specifications and Parameters

Biological Background: C1qa Function and Localization

• Complement C1q subcomponent subunit A (C1qa) is a core component of the C1 complex, which initiates the classical pathway of the complement system, linking innate and adaptive immunity.
• C1qa, together with C1qB and C1qC, specifically binds the Fc regions of IgG or IgM complexed with antigens, triggering the proteolytic cascade that leads to pathogen opsonization and clearance.
• Beyond immune-complex recognition, the C1q subcomplex also binds phosphatidylserine exposed on apoptotic cells, potentially promoting their silent clearance via complement activation.
• C1qa is secreted and can associate with the cell surface, positioning it at the interface of humoral and cellular immune responses.
• The protein contains a collagen-like domain and a globular C1q domain, with structural features including calcium-binding sites, disulfide bonds, glycosylation, and hydroxylation modifications crucial for its function.
• The classical complement pathway initiated by C1qa is activated by a hexamer of IgG or a pentamer of IgM, underscoring the importance of multivalent binding for efficient activation.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 23–245 of mouse C1qa, covering the mature secreted protein; confirm reactivity with both pro- and processed forms in your experimental system.
• For Western blotting, the predicted molecular weight is ~26 kDa under reducing conditions; consider using non-reducing gels to assess multimeric complex formation if investigating native C1q assembly.
• In immunohistochemistry (IHC-P), antigen retrieval may be required for optimal detection; validate with appropriate positive and negative control tissues.
• Immunofluorescence (IF/ICC) may detect cell surface-associated C1qa; optimize fixation and permeabilization conditions to preserve epitope accessibility.
• This antibody is reported to cross-react with human and rat C1qa; always confirm species specificity in your assays, particularly when using tissues with varying endogenous immunoglobulin levels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including this anti-C1qa polyclonal antibody for complement system and innate immunity research. From validated antibody pairs and optimized polyclonal antibodies to bulk ancillary reagents, CamelBio streamlines procurement for every stage of assay development. For rare or custom targets, our sourcing network ensures reliable access to specialized materials, accelerating your journey from concept to clinic.