Anti-C1orf146/SPO16 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - Q3KQP7

Core Product Specifications and Parameters

Biological Background: SPO16/C1orf146 Function and Localization

• SPO16 (also known as SCRE or C1orf146) is a 21 kDa protein encoded by the Spo16 gene in mouse. It is a key component of the synaptonemal complex central element, essential for meiotic chromosome dynamics.
• It reinforces the central element integrity, thereby stabilizing the synaptonemal complex and ensuring proper meiotic prophase I progression in both male and female germ cells. Related references: PMID:30949703
• SPO16 promotes homologous recombination and crossing-over by associating with SHOC1; it is required for the localization of TEX11 and MSH4 to recombination intermediates. Related references: PMID:30746471
• Subcellularly, SPO16 localizes to chromosomes, consistent with its role in meiotic chromosome structure.
• Protein expression is detected specifically in testis, indicating a pivotal role in spermatogenesis.
• Keywords including Alternative splicing, Meiosis, and Reference proteome highlight its regulated expression and critical function in meiosis.

Experimental Guidance and Technical Tips

• Based on the immunogen design, this antibody is expected to recognize the SPO16 protein fragment spanning amino acids 47-173. For WB, a band around 21 kDa may be observed; validate with appropriate controls such as testis lysates from wild-type versus knockout animals if available.
• For IF-P, testis sections are recommended and antigen retrieval conditions may need optimization.
• For ELISA, consider using the recombinant immunogen as a coating antigen.
• Note that alternative splicing may produce multiple isoforms; confirm specificity in your system.

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CamelBio is your one-stop sourcing bridge for high-quality IVD raw materials. From validated antibody pairs to optimized polyclonal antibodies like this Anti-C1orf146/SPO16 antibody, we support diagnostic manufacturers and researchers with reliable reagents for meiosis and fertility studies. Our extensive catalog and custom sourcing services cover bulk ancillary reagents and rare target raw materials, streamlining your development from concept to clinic.