Anti-C1GALT1C1 Rabbit Polyclonal Antibody for WB and ELISA - Q96EU7

Core Product Specifications and Parameters

Biological Background: C1GALT1C1 Function and Localization

• C1GALT1C1 (also known as COSMC, C1Gal-T2, C38H2-L1) is a ubiquitously expressed molecular chaperone essential for the biosynthesis of O-glycans, specifically the T antigen (Gal-beta1-3GalNAc-alpha1-Ser/Thr).
• It acts by assisting the folding and stability of core 1 beta-3-galactosyltransferase (C1GALT1), enabling the generation of core 1 O-glycan structures that serve as precursors for many extended O-glycans in glycoproteins.
• The protein is a membrane-associated chaperone with a signal-anchor and a predicted transmembrane helix, consistent with its localization at the endoplasmic reticulum/Golgi interface where O-glycosylation occurs.
• Tissue profiling shows abundant expression in salivary gland, stomach, small intestine, kidney, and testis, with moderate levels in brain, cerebellum, thymus, spleen, lung, pancreas, ovary, and uterus.
• Mutations in C1GALT1C1 are linked to disease variants, including atypical hemolytic uremic syndrome, highlighting its importance in glycoprotein quality control.
• The UniProt entry lists it under keywords: Chaperone, Disease variant, Hemolytic uremic syndrome, Membrane, Signal-anchor, Transmembrane, Transmembrane helix, and Reference proteome.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 30–190) is a recombinant fusion protein; the antibody is expected to recognize the native C1GALT1C1 protein. Validate specificity in relevant sample systems.
• For western blotting, a band at approximately 36 kDa should be observed; confirm with knock-down or overexpression models to ensure band identity.
• In ELISA applications, titrate the antibody against the full-length or immunogen fragment to establish an optimal working dilution.
• Cross-reactivity with mouse and rat has been confirmed; verify detection in lysates from these species if used.
• As a polyclonal antibody, batch-to-batch consistency should be assessed for critical quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers with validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for O-glycosylation research. Explore our full portfolio to accelerate your immunoassay development, from concept to clinic. We also offer rare target raw-material sourcing and technical consulting to streamline your IVD workflow.