Anti-C19orf10 Polyclonal Antibody for WB, IHC-P, ELISA - Q969H8

Core Product Specifications and Parameters

Biological Background: C19orf10/MYDGF Function and Localization

• C19orf10 encodes myeloid-derived growth factor (MYDGF), a secreted protein also known as IL25, IL27, SF20, and IL27w.
• MYDGF promotes cardiac myocyte survival and adaptive angiogenesis after myocardial infarction (MI). It stimulates endothelial cell proliferation via MAPK1/3, STAT3, and CCND1 signaling and is involved in angiogenesis. Related references: PMID:25581518
• It inhibits cardiac myocyte apoptosis through a PI3K/AKT-dependent pathway (By similarity).
• Subcellularly, MYDGF localizes to the secretory pathway: secreted, endoplasmic reticulum-Golgi intermediate compartment, endoplasmic reticulum, and Golgi apparatus.
• Expressed in eosinophils (PubMed:29954947), bone marrow (PubMed:25581518), and synovial tissue; found in synovial fluid of patients with arthropathies (PubMed:17362502). Related references: PMID:29954947 PMID:25581518 PMID:17362502
• Annotated keywords: angiogenesis, apoptosis, direct protein sequencing, reference proteome, signal.
• Calculated molecular weight: 18795 Da.

Experimental Guidance and Technical Tips

• The immunogen is a full-length recombinant protein (amino acids 1-173), which covers the entire MYDGF precursor; this design may favor detection of both precursor and mature forms.
• For Western blot (WB), a band around 19 kDa is expected; consider using a 12-15% SDS-PAGE gel for optimal resolution.
• For immunohistochemistry (IHC-P), antigen retrieval may be necessary; validate staining in relevant tissue sections (e.g., bone marrow, synovium).
• For ELISA, the antibody can be used as capture or detection reagent; pairing with another clone or a matched pair is recommended for sandwich ELISA.
• Since cross-reactivity is reported for human, mouse, and rat, verify specificity in the target species' sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. Our one-stop sourcing bridge supports diagnostic manufacturers and researchers from concept to clinic, with expertise in cardiac biomarkers and angiogenic targets like MYDGF. Whether you need rare-target antibodies or customized raw materials, CamelBio streamlines procurement to accelerate your development.