Anti-C14orf166/RTRAF Rabbit Polyclonal Antibody for WB, ELISA - Q9Y224

Core Product Specifications and Parameters

Biological Background: RTRAF (C14orf166) Function and Localization

• Protein identity: RTRAF (RNA transcription, translation and transport factor protein) is the official name for the C14orf166 gene product. It is also known as CLE7 homolog (CLE, hCLE) and functions as an accessory subunit of the tRNA-splicing ligase complex.
• Core RNA processing role: RTRAF directly participates in joining spliced tRNA halves to produce mature tRNAs, incorporating the splice junction phosphate into the canonical 3',5'-phosphodiester bond. Related references: PMID:21311021 PMID:24870230
• Transcriptional regulation: The protein modulates mRNA transcription by interacting with RNA Polymerase II, influencing gene expression control. Related references: PMID:16950395
• RNA transport involvement: RTRAF is implicated in the intracellular transport of RNA molecules, potentially facilitating their movement between nucleus and cytoplasm. Related references: PMID:24608264
• Subcellular distribution: The protein localizes to multiple compartments including the nucleus, cytoplasm, cytosol, perinuclear region, centrosome, and microtubule organizing center, reflecting its diverse functions.
• Tissue expression pattern: It is broadly expressed across tissues, with highest levels in heart and skeletal muscle, intermediate in liver and pancreas, and weak in adult brain and lung. Notably, overexpression is observed in many brain tumors.
• Post-translational modification and viral interaction: RTRAF is subject to acetylation. During influenza A virus infection, it is hijacked to support viral replication. Related references: PMID:21900157

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human protein (aa 1-244). Given its high sequence conservation, cross-reactivity with mouse is expected; verify reactivity in your specific experimental system if using other species.
• Western blot analysis should detect a band around 28 kDa. Optimize blocking and antibody dilution empirically using a positive control lysate from human or mouse cells known to express RTRAF (e.g., HeLa, HEK293, or cardiac/skeletal muscle tissues).
• For ELISA, consider using the recombinant immunogen as a coating antigen and titrate the antibody to establish a robust standard curve. Pair with a suitable secondary antibody for detection.
• The protein's multi‑compartment localization and involvement in tRNA splicing suggest that fractionation or immunofluorescence could be informative; however, these applications are not guaranteed and require validation.

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