Anti-C12orf10 Rabbit Polyclonal Antibody for IHC-P and ELISA - Q9HB07

Core Product Specifications and Parameters

Biological Background: MYG1 exonuclease Function and Localization

• MYG1 (also known as C12orf10) is a 3'-5' RNA exonuclease that processes RNA in both the nucleus and mitochondrion, playing a key role in nucleo-mitochondrial crosstalk. Related references: PMID:31081026
• In the nucleolus, it processes pre-ribosomal RNA, contributing to ribosome assembly and affecting cytoplasmic translation.
• In the mitochondrial matrix, it processes the 3'-termini of mitochondrial ribosomal and messenger RNAs, controlling mitochondrial protein synthesis.
• Subcellular localization includes the nucleoplasm, nucleolus, and mitochondrial matrix, reflecting its dual organellar function.
• Ubiquitously expressed, with highest levels in testis, suggesting a role in energy-demanding tissues.
• Contains a transit peptide and undergoes post-translational modifications such as acetylation and phosphorylation (based on proteomics identification).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 48–376 of human C12orf10, covering a large portion of the protein. This broad region may enable recognition of different conformational states; consider testing performance in both native and denaturing conditions if adapting to additional assay formats.
• For IHC-P, optimize antigen retrieval conditions and primary antibody dilution. Validate staining with appropriate positive controls (e.g., testis tissue) and negative controls.
• ELISA can be employed for quantitative detection or screening; pair with a suitable anti-rabbit secondary antibody and consider cross-reactivity of the detection system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-C12orf10 polyclonal antibody supports investigations into RNA processing and nucleo-mitochondrial communication, helping customers advance their research and development pipelines. Contact CamelBio to explore tailored sourcing solutions for rare targets and complete reagent panels.