Anti-C10orf32 Polyclonal Antibody for WB, IF/ICC, ELISA - Q96B45

Core Product Specifications and Parameters

Biological Background: BORCS7/C10orf32 Function and Localization

• BORCS7 (C10orf32), also known as BLOC-1-related complex subunit 7 or Diaskedin, is a 105 amino acid protein encoded by the BORCS7 gene on chromosome 10.
• It serves as a subunit of the BORC (BLOC-1-related complex) which associates with the cytosolic face of lysosomes.
• The BORC complex recruits the small GTPase ARL8B, thereby coupling lysosomes to microtubule plus-end-directed kinesin motors and facilitating lysosome movement toward the cell periphery.
• This lysosome positioning function is critical for intracellular trafficking, nutrient sensing, and cellular signaling.
• BORCS7 is anchored to the lysosome membrane, consistent with its role in a membrane-associated multiprotein complex.
• UniProt annotation highlights its involvement in lysosome biology, membrane dynamics, and reference proteome completeness.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant fusion protein covering the full-length human C10orf32 (aa 1–105). Owing to its polyclonal nature, it is expected to recognize multiple epitopes along the N-terminal region.
• For Western blot, consider using reducing conditions and a 15% SDS-PAGE gel to resolve the ~12 kDa target. Validate the band pattern in relevant human or rat cell lysates.
• In immunofluorescence/immunocytochemistry (IF/ICC), co-staining with a lysosomal marker (e.g., LAMP1) is advisable to confirm lysosomal localization. Permeabilization and fixation conditions should be optimized for each cell type.
• For ELISA, titrate the antibody as both capture and detection reagent to define optimal working concentrations. Pairing with a monoclonal anti-BORCS7 may improve assay specificity.
• Since cross-reactivity is documented for human and rat, testing in mouse or other species is not recommended without preliminary sequence alignment and experimental validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. This polyclonal antibody against C10orf32 (BORCS7) addresses targets in lysosome biology and intracellular trafficking—areas increasingly relevant to neurodegenerative and lysosomal storage disorders. Whether you require validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, or rare target raw-material sourcing, our integrated supply chain can meet your project needs from concept to clinic.