Anti-C10orf2/Twinkle mtDNA Helicase Polyclonal Antibody for WB, ELISA - Q96RR1

Core Product Specifications and Parameters

Biological Background: Twinkle mtDNA Helicase Function and Localization

• Essential mitochondrial helicase involved in mtDNA replication and repair. It drives DNA strand separation to form processive replication forks for leading strand synthesis. Related references: PMID:12975372 PMID:15167897 PMID:17324440 PMID:18039713
• Participates in mtDNA repair, including dissociation of invading strands from immobile or mobile D-loop structures irrespective of single-strand polarity. Related references: PMID:27226550
• Forms a replisome complex with DNA polymerase gamma (POLG) and mitochondrial single-stranded DNA-binding protein (mtSSB). Preferentially unwinds DNA substrates containing pre-existing 5'- and 3'- single-stranded tails and 5'-flap structures. Related references: PMID:12975372 PMID:15167897 PMID:18039713
• Exhibits DNA strand annealing, strand-exchange, and branch migration activities in addition to helicase function. Related references: PMID:22383523 PMID:26887820 PMID:27226550
• Localizes to the mitochondrial matrix, nucleoids, and inner membrane, consistent with its role in mtDNA maintenance.
• Tissue expression is highest in skeletal muscle, testis, and pancreas, with lower levels in heart, brain, liver, kidney, and other tissues. Expression is co-regulated with MRPL43.
• Alternative splicing produces an isoform lacking DNA unwinding and ATP hydrolysis activities, and unable to bind single- or double-stranded DNA. Related references: PMID:18039713
• Associated with multiple mitochondrial disorders, including progressive external ophthalmoplegia (PEO) and mitochondrial DNA depletion syndrome, highlighting its clinical significance.

Experimental Guidance and Technical Tips

• The immunogen maps to amino acids 585–684, a region likely conserved in mouse (indicated by cross-reactivity). For Western blot, anticipate a band near 77 kDa; confirm detection using appropriate positive and negative controls in your sample system.
• For ELISA applications, consider employing the immunizing peptide as a coating antigen or competition control to verify antibody specificity.
• As a polyclonal reagent, lot-to-lot consistency may vary; it is advisable to qualify each new batch in your assay before large-scale use.
• Due to Twinkle’s intramitochondrial localization, ensure efficient mitochondrial enrichment or lysis protocols when preparing samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies procurement for diagnostic manufacturers and research laboratories by providing end-to-end access to IVD raw materials. This anti-Twinkle polyclonal antibody supports mitochondrial biology research, and we complement such tools with validated antibody pairs, bulk ancillary reagents, and rare-target sourcing capabilities. Our integrated supply chain covers every stage from concept to clinic, helping you accelerate assay development.