Anti-C-Reactive Protein (CRP) Polyclonal Antibody for WB, IF/ICC, ELISA - P02741

Core Product Specifications and Parameters

Biological Background: C-Reactive Protein Function and Localization

• C-reactive protein (CRP), also known as PTX1, is an acute-phase protein secreted into plasma. (Gene: CRP)
• It plays a key role in host defense by promoting agglutination, bacterial capsular swelling, phagocytosis, and complement fixation via calcium-dependent binding to phosphorylcholine.
• CRP can bind to DNA and histones, potentially scavenging nuclear material from damaged cells.
• It is a secreted protein found predominantly in plasma.
• Key structural features include a signal peptide, disulfide bonds, calcium-binding sites, and pyrrolidone carboxylic acid modification.
• As a classic acute-phase reactant, CRP levels rise dramatically in inflammation and infection, serving as a critical biomarker.
• Alternative splicing of the CRP gene has been noted, though its functional significance may vary.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 100–200 of human CRP (NP_000558.2); verify epitope specificity in your experimental context.
• For WB, the predicted molecular weight is ~25 kDa (UniProt), but observe ~23 kDa per product data; validate in the relevant sample system.
• Cross-reactivity with mouse and rat is reported; consider sequence homology in the immunogen region when using these species.
• For IF/ICC, optimize fixation and permeabilization protocols as CRP is secreted.
• In ELISA applications, pairing with a suitable capture antibody is recommended; validate performance with appropriate standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For CRP-related research and assay development, we supply validated antibody pairs, optimized polyclonal antibodies like this CRP pAb, and bulk ancillary reagents. Our sourcing capabilities also cover rare-target raw materials to support your IVD pipeline.