Anti-C-Reactive Protein (CRP) Polyclonal Antibody for WB, ELISA - P02741

Core Product Specifications and Parameters

Biological Background: C-Reactive Protein (CRP) Function and Localization

• C-reactive protein (CRP) is an acute-phase protein belonging to the pentraxin family, encoded by the CRP gene (also known as PTX1).
• CRP promotes agglutination, bacterial capsular swelling, phagocytosis, and complement fixation through calcium-dependent binding to phosphorylcholine.
• It can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
• The protein is secreted into plasma, with expression induced primarily by IL-6 during inflammation; circulating levels increase dramatically in response to acute-phase stimuli.
• CRP contains calcium-binding sites and a disulfide bond, and alternative splicing has been reported.
• Post-translational modifications include a pyrrolidone carboxylic acid at the N-terminus after signal peptide cleavage.
• The mature protein has a calculated molecular weight of approximately 25 kDa, but is often detected around 23 kDa due to potential modifications.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa 1-50) of human CRP; this region is expected to be exposed in denaturing conditions suitable for western blotting.
• For western blot, consider using reduced and denatured human plasma or recombinant CRP as positive controls; the predicted band is approximately 23-25 kDa.
• For ELISA, pairing this antibody with a detection antibody targeting a non-overlapping epitope (e.g., C-terminal region) may improve sensitivity; validate the pair in clinically relevant matrices.
• Due to CRP's acute-phase nature, sample levels can vary widely; include appropriate calibrators and normal range controls in your assay.
• Mouse cross-reactivity is reported; validate in mouse serum or plasma to ensure specificity if using samples from mouse models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides integrated sourcing solutions for IVD raw materials, supporting your CRP immunoassay development from target selection to clinical translation. In addition to high-quality anti-CRP antibodies, we offer validated antibody pairs, bulk ancillary reagents, and custom sourcing services for rare biomarker targets. Partner with us to streamline your diagnostic kit development workflow and ensure reliable supply chain continuity.