Anti-c-Myc Polyclonal Antibody for WB, ELISA - P01106

Core Product Specifications and Parameters

Biological Background: c-Myc Function and Localization

• Proto-oncogene transcription factor, also known as bHLHe39, c-Myc, or transcription factor p64, encoded by the MYC gene. Functions as a class E basic helix-loop-helix protein.
• Binds DNA in a non-specific manner and specifically recognizes the E-box motif 5'-CAC[GA]TG-3' (PubMed:24940000, PubMed:25956029). Activates transcription of growth-related genes and promotes VEGFA production to drive sprouting angiogenesis.
  Related references:
  PMID:24940000
  PMID:25956029
• Plays a critical role in somatic cell reprogramming and maintenance of embryonic stem cell self-renewal (By similarity).
• Regulates RNA polymerase II pause release together with TAF6L to activate target gene expression (By similarity).
• Modulates alternative splicing: positively regulates HNRNPA1, HNRNPA2, and PTBP1, which repress exon 9 inclusion in PKM, leading to exon 10 inclusion and production of the PKM M2 isoform (PubMed:20010808).
  Related references:
  PMID:20010808
• Localizes to the nucleus (nucleoplasm and nucleolus), and can also be found in the cytoplasm and associated with chromosomes.
• Subject to various post-translational modifications including acetylation, phosphorylation, glycosylation, and ubiquitin-like conjugation (isopeptide bond). Keywords: Phosphoprotein, Glycoprotein, Ubl conjugation.
• Frequently involved in chromosomal rearrangements in cancers, functioning as a potent proto-oncogene.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (amino acids 347-439) of human c-Myc; due to high sequence homology, cross-reactivity with mouse is expected, but validate in the relevant sample system.
• For Western blot (WB), consider starting with a dilution range of 1:500-1:2000; optimal working concentration must be determined empirically.
• c-Myc is a short-lived protein; include protease inhibitors during lysis and use appropriate controls to detect specific bands.
• For ELISA, titrate the antibody against the immunogen peptide or full-length protein to establish standard curves.
• Polyclonal antibodies may exhibit batch-to-batch variability; re-validate critical experiments when switching lots.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, from validated antibody pairs and optimized polyclonal antibodies to bulk ancillary reagents. Whether sourcing rare targets like c-Myc for oncology research or scaling up production, CamelBio bridges supply chain gaps efficiently. We partner with you from concept to clinic, offering reliable raw-material solutions for your diagnostic development needs.