Anti-c-Jun Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P05412

Core Product Specifications and Parameters

Biological Background: c-Jun Function and Localization

• c-Jun is a transcription factor and a core component of the Activator Protein-1 (AP-1) complex, which recognizes the consensus motif 5'-TGA[GC]TCA-3' and regulates gene expression in response to various stimuli (PubMed:10995748, PubMed:22083952). Related references: PMID:10995748 PMID:22083952
• Heterodimerization with FOS family proteins enhances DNA binding and transcriptional activity of the AP-1 complex, playing critical roles in cell proliferation, differentiation, and apoptosis.
• c-Jun contributes to activation-induced cell death (AICD) of T cells by binding to the AP-1 site of the FASLG/CD95L promoter and inducing its transcription upon TCR/CD3 signaling (PubMed:12618758). Related references: PMID:12618758
• Phosphorylation by HIPK3 promotes the activity of NR5A1, leading to increased steroidogenic gene expression in response to cAMP signaling (PubMed:17210646). Related references: PMID:17210646
• In colorectal cancer, activated KRAS-mediated transcriptional activation of USP28 involves c-Jun binding to the USP28 promoter, highlighting its oncogenic role (PubMed:24623306). Related references: PMID:24623306
• As a proto-oncogene, c-Jun is subject to multiple post-translational modifications including phosphorylation, acetylation, and ubiquitination, which regulate its stability and activity.
• It localizes predominantly to the nucleus, consistent with its role as a transcription factor.
• c-Jun is expressed in developing and adult prostate, as well as prostate cancer cells, and can be hijacked during Epstein-Barr virus infection to activate viral gene expression.

Experimental Guidance and Technical Tips

• The immunogen (aa 90–230) spans a region critical for DNA binding and dimerization; consider testing antibody reactivity against relevant post-translational modifications or variants.
• For western blotting, a band near 36 kDa is expected; however, extensive phosphorylation may cause slight shifts in migration. Optimize blocking and antibody dilution for target cell lines.
• In immunofluorescence/ICC, a predominantly nuclear signal is anticipated. Include appropriate controls (e.g., knockout or knockdown) to confirm specificity.
• Cross-reactivity with mouse c-Jun is reported; verify reactivity in your experimental model if using other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides integrated access to premium IVD raw materials, including this c-Jun polyclonal antibody, supporting research in oncology, transcriptional regulation, and AP-1 signaling. Our portfolio encompasses validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents for bulk procurement. Leverage our sourcing expertise to accelerate assay development with rare-target and custom raw-material solutions.