Anti-c-Jun Rabbit Polyclonal Antibody for WB and ELISA - P05412

Core Product Specifications and Parameters

Biological Background: c-Jun Function and Localization

• c-Jun (AP-1, p39) is a transcription factor and a core component of the AP-1 complex, belonging to the basic leucine zipper (bZIP) family.
• It recognizes and binds the AP-1 consensus motif 5'-TGA[GC]TCA-3' to regulate gene expression (PubMed:10995748, PubMed:22083952). Related references: PMID:10995748 PMID:22083952
• Heterodimerizes with FOS family proteins to form an active AP-1 transcription complex, enhancing DNA binding and transcriptional activity (By similarity).
• Plays a role in activation-induced cell death of T cells by binding to the FASLG/CD95L promoter and inducing its transcription (PubMed:12618758). Related references: PMID:12618758
• Promotes NR5A1 activity when phosphorylated by HIPK3, leading to increased steroidogenic gene expression upon cAMP signaling (PubMed:17210646). Related references: PMID:17210646
• Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer cells (PubMed:24623306). Related references: PMID:24623306
• Localizes predominantly to the nucleus and is expressed in the developing and adult prostate as well as prostate cancer cells.
• Post-translationally modified by phosphorylation and acetylation, which regulate its transcriptional activity.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1–70) of c-Jun; ensure the epitope is accessible in your assay format.
• For Western blot, the predicted molecular weight is 36 kDa; consider that post-translational modifications may cause band shifts.
• This antibody shows cross-reactivity with mouse and rat, but validation in your specific experimental system is recommended.
• ELISA applications may require optimization of coating conditions and detection reagents.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive sourcing solutions for IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. Whether you need validated antibody pairs, bulk polyclonal antibodies like this anti-c-Jun pAb, or ancillary reagents for AP-1 pathway and cancer studies, we streamline procurement of high-quality raw materials. Our capabilities also include sourcing rare targets and offering tailored technical consulting to accelerate your assay development.