Anti-c-Fos Rabbit Polyclonal Antibody for WB, IP, ELISA - P01100

Core Product Specifications and Parameters

Biological Background: c-Fos Function and Localization

• Protein c-Fos is a nuclear phosphoprotein, also known as the AP-1 transcription factor subunit, Fos proto-oncogene, G0/G1 switch regulatory protein 7, or cellular oncogene fos.
• It forms a tight, non-covalent complex with JUN/AP-1, and the heterodimer binds symmetric DNA half-sites to regulate transcription of target genes.
• Upon TGF-beta activation, c-Fos assembles into a multimeric SMAD3/SMAD4/JUN/FOS complex at AP1/SMAD-binding sites, modulating TGF-beta-mediated signaling cascades.
• c-Fos plays a critical role in the development of cells destined to form and maintain the skeleton and is essential for signal transduction, cell proliferation, and differentiation.
• In growing cells, c-Fos activates phospholipid synthesis by possibly stimulating CDS1 and PI4K2A; this activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.
• Subcellular localization includes the nucleus, endoplasmic reticulum, cytoplasm, and cytosol, reflecting its diverse regulatory functions.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant fusion protein spanning the N‑terminus (aa 1–114) of human c‑Fos. For detection of endogenous c‑Fos, consider validating expression in appropriate cell lines or tissue samples, as c‑Fos is an immediate‑early gene rapidly induced by various stimuli.
• In Western blot, an approximately 41 kDa band is expected; however, post‑translational modifications (e.g., phosphorylation) may cause band shifts or multiple bands.
• For immunoprecipitation, standard protocols can be applied. Note that c‑Fos naturally forms heterodimers with JUN family members, so co‑immunoprecipitation of binding partners may be observed.
• ELISA assays may require optimization of coating antigen and antibody dilution. A recombinant c‑Fos protein fragment corresponding to the immunogen is suggested as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated sourcing partner for IVD raw materials, providing comprehensive support from concept to clinic. For c‑Fos and AP‑1 transcription factor research, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to streamline your assay development. Our global sourcing network also efficiently delivers rare‑target raw materials, ensuring you have reliable access to the components needed for diagnostic innovation.