Anti-c-Fos Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P01100

Core Product Specifications and Parameters

Biological Background: c-Fos Function and Localization

• Protein c-Fos is a nuclear phosphoprotein and a core component of the AP-1 transcription factor complex, forming a tight heterodimer with JUN family proteins to regulate gene expression.
• It plays a pivotal role in signal transduction, cell proliferation, and differentiation, including mediating TGF-beta signals through a SMAD3/SMAD4/JUN/FOS complex at AP1/SMAD-binding sites.
• c-Fos is critically involved in skeletal development and maintenance, influencing the differentiation of osteoblasts and chondrocytes.
• In growing cells, c-Fos can activate phospholipid synthesis by potentially stimulating CDS1 and PI4K2A, an activity requiring tyrosine dephosphorylation and association with the endoplasmic reticulum.
• Subcellularly, c-Fos localizes predominantly to the nucleus but is also found in the endoplasmic reticulum, cytoplasm, and cytosol.
• Multiple isoforms exist due to alternative splicing, and the protein undergoes various post-translational modifications, including phosphorylation and ubiquitination.
• Known aliases include p55, AP-1, C-FOS, and G0/G1 switch regulatory protein 7 (G0S7).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 211–281 of human c-Fos, a region conserved across species, which aligns with the observed reactivity with mouse and rat orthologs.
• For Western blot, the predicted molecular weight is ~41 kDa; validate the specific band in relevant cell or tissue lysates, including appropriate positive and negative controls (e.g., stimulated vs. unstimulated cells).
• In immunofluorescence and immunohistochemistry, optimize fixation and permeabilization protocols as c-Fos is primarily nuclear but may also exhibit cytoplasmic staining.
• For ELISA applications, titrate the antibody and confirm performance in your specific assay system, considering its use as either a capture or detection reagent.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, including validated antibody pairs and optimized monoclonal antibodies like this c-Fos product. Beyond antibodies, we offer bulk ancillary reagents and can source rare-target raw materials, supporting your workflow from concept to clinic. For your c-Fos-related studies in oncology or developmental biology, rely on CamelBio as your integrated sourcing partner.