Anti-bZIP46 Polyclonal Antibody for WB, ELISA - Q69TW5

Core Product Specifications and Parameters

Biological Background: bZIP46 Function and Localization

• bZIP46, also known as OsAREB8, OsABF2, OsABF3, and ABI5-LIKE 1, is a basic leucine zipper (bZIP) transcription factor in rice (Oryza sativa).
• It functions as a key component in abscisic acid (ABA) signaling; its transcription factor activity is fully activated by ABA treatment.
  Related references:
  PMID:19947981, PMID:26300907, PMID:27468891
• bZIP46 positively regulates the expression of abiotic stress-responsive genes through an ABA-dependent signaling pathway, thereby enhancing drought stress tolerance.
  Related references:
  PMID:20576316, PMID:22301130
• The protein is involved in crosstalk between ABA and auxin signaling: it directly binds to ABA-responsive elements (ABREs) in target gene promoters, notably WRKY family genes, and suppresses auxin signaling by targeting ABRE-containing genes related to auxin metabolism or signaling.
  Related references:
  PMID:21546455
• bZIP46 localizes to the nucleus, consistent with its role as a transcription factor.
• It is widely expressed in various tissues, including roots, shoots, leaves, flag leaves, stems, flowers, and panicles.
  Related references:
  PMID:20576316, PMID:21546455
• As a member of the AREB/ABF family, bZIP46 contains a conserved bZIP domain required for DNA binding and dimerization; it is classified under stress response and transcription regulation keywords.

Experimental Guidance and Technical Tips

• The immunogen comprises a recombinant protein fragment (amino acids 100–200), which spans a central region of bZIP46. For Western blot (WB), we recommend using whole-cell lysates from Oryza sativa tissues, with detection expected at approximately 35 kDa. Due to potential post-translational modifications, the observed band may shift slightly.
• For ELISA, consider using purified recombinant bZIP46 or bZIP46-derived peptides as coating antigens. Include appropriate negative and positive controls to validate assay specificity.
• As a polyclonal antibody, lot-to-lot variability may occur; we recommend titrating the antibody for each new application and validating performance in the relevant sample system.
• Cross-reactivity is reported for Oryza sativa; testing in other plant species may require sequence homology analysis of the immunogen region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. This anti-bZIP46 antibody is suitable for plant stress biology and ABA signaling studies, and we offer complementary reagents such as validated antibody pairs, antigen standards, and ancillary detection reagents. For custom or rare-target sourcing, CamelBio provides tailored procurement and technical consulting services.