Anti-BYSL Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q13895

Core Product Specifications and Parameters

Biological Background: BYSL Function and Localization

• Bystin (encoded by BYSL/ENP1 gene) is a 50 kDa protein involved in ribosome biogenesis and cell adhesion.
• Essential for processing of 20S pre-rRNA precursor into mature 40S ribosomal subunits.
• May mediate trophinin-dependent cell adhesion during human embryo implantation.
• Localizes to the cytoplasm, nucleus, and nucleolus, consistent with its role in ribosomal processing.
• Expressed in placenta from week 6 of pregnancy, predominantly in syncytiotrophoblast and endometrial decidual cells; levels decline after week 10.
• Post-translational modifications include phosphorylation and methylation (UniProt keyword annotations).
• Identified in proteomics screens, supporting its role in fundamental cellular processes (Reference proteome).

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 120-280) of human BYSL; the antibody may recognize both full-length protein and potential isoforms.
• For western blotting, the predicted band size is ~50 kDa; consider using positive control lysates from HeLa cells or placental tissue.
• In paraffin-embedded immunohistochemistry, antigen retrieval may be required; validate with relevant tissue sections such as first-trimester placenta.
• ELISA performance should be optimized with appropriate coating antigen and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers one-stop access to IVD raw materials, including validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents. For researchers studying ribosome biogenesis or placental development, we can source rare targets like bystin antibodies and provide technical support from concept to clinic. Partner with us for efficient procurement and expert consultation.