Anti-BVES (POPDC1) Polyclonal Antibody for WB and ELISA - Q8NE79

Core Product Specifications and Parameters

Biological Background: BVES Function and Localization

• BVES (Popeye domain-containing protein 1, also known as POPDC1) is a cell adhesion molecule that plays a key role in establishing and maintaining cell integrity. It is involved in the formation and regulation of tight junction (TJ) paracellular permeability in epithelial cells. Related references: PMID:16188940
• The protein interacts with VAMP3, modulating VAMP3-mediated vesicular transport and the recycling of various receptor molecules. It also regulates cell shape and movement by modulating Rho-family GTPase activity through interaction with ARHGEF25/GEFT.
• BVES induces primordial adhesive contact and aggregation of epithelial cells in a Ca²⁺-independent manner. It is also implicated in striated muscle regeneration, repair, and the regulation of cell spreading (By similarity).
• In cardiac muscle, BVES is essential for maintaining cardiac function. It regulates heart rate dynamics, at least in part, through cAMP-binding and by enhancing cell surface expression of the potassium channel KCNK2, thereby increasing current density. Related references: PMID:26642364
• BVES localizes to the lateral cell membrane, tight junctions, sarcolemma, and caveolae. Its caveolae association is critical for preserving the structural and functional integrity of caveolae and protecting the heart against ischemia injury.
• Tissue-specific expression is observed in epithelial cells (at the protein level), fetal and adult heart, and skeletal muscle.
• The protein is classified under keywords including cAMP-binding, cell adhesion, tight junction, lim-girdle muscular dystrophy, and transmembrane. It contains a Popeye domain and is a glycoprotein and phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 241-360) of human BVES. This region may provide good antigenicity; researchers should consider its accessibility in native versus denaturing conditions.
• For Western blot (WB), a band at approximately 41 kDa is expected. Include appropriate positive controls, such as lysates from human heart or skeletal muscle tissue, or epithelial cell lines known to express BVES.
• In ELISA applications, optimize coating concentration and block non-specific binding carefully. Recombinant BVES protein or cell lysates with confirmed expression can be used as target antigens.
• Due to the role of BVES in tight junctions and caveolae, subcellular fractionation or specific lysis conditions may improve detection. Validate the antibody‘s performance in the relevant cellular context.
• Cross-reactivity has been verified for human; if using in other species, confirm sequence homology and perform preliminary validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to serving diagnostic manufacturers, laboratories, and research institutes as a comprehensive one-stop supplier of IVD raw materials. Leveraging our expertise in antibody development and protein biochemistry, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing of rare-target raw materials. This anti-BVES polyclonal antibody, targeting a protein critical to tight junction biology and cardiac function, exemplifies the specialized reagents we can provide to support your research and development needs. Contact us to discuss how we can help streamline your supply chain from concept to clinic.