Anti-Butyrylcholinesterase Monoclonal Antibody for WB and ELISA - P06276

Core Product Specifications and Parameters

Biological Background: Butyrylcholinesterase Function and Localization

• Butyrylcholinesterase (BChE), also known as pseudocholinesterase or choline esterase II, is a secreted serine esterase with broad substrate specificity.
• It hydrolyzes acetylcholine and other choline esters, contributing to the inactivation of the neurotransmitter acetylcholine.
• BChE additionally degrades neurotoxic organophosphate esters, playing a detoxifying role.
• The protein is a glycoprotein containing disulfide bonds and sialic acid modifications.
• Subcellular localization: Secreted into the blood plasma.
• Tissue specificity: Detected in blood plasma at the protein level and present in most cells except erythrocytes.
• Encoded by the BCHE gene (CHE1), it belongs to the cholinesterase family.
• The canonical isoform has a predicted molecular weight of approximately 68 kDa.

Experimental Guidance and Technical Tips

• The immunogen sequence targets the C-terminal region (amino acids 500–602) of human BChE, suitable for detecting the full-length protein in applications like WB and ELISA.
• For Western blot, the predicted molecular weight is 68 kDa; use appropriate molecular weight markers and consider validating with positive controls such as plasma or serum samples.
• In ELISA, optimize coating and detection conditions as needed for this rabbit monoclonal antibody, and confirm linearity and specificity using recombinant protein or known samples.
• Cross-reactivity is confirmed with human; if using other species, verify reactivity experimentally due to potential sequence variations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering high-quality antibodies like this BChE monoclonal antibody for neurobiology and toxicology research. Our supply chain includes validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support your diagnostic development from concept to clinic.